Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora

A psychrophilic microorganism and cold-soaked field technology, applied in the field of microorganisms, can solve the problems of slow mineralization rate, low activity and low nutrient availability of organic matter, so as to accelerate the decomposition of organic matter, activate nutrients in soil reservoirs, and promote crop growth. Effect

Inactive Publication Date: 2015-07-08
GUANGZHOU XINNONGKE FERTILIZER SCI & TECH
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Problems solved by technology

How to develop psychrophilic microbial flora products that can quickly mineralize soil organic matter and significantly activate soil effective nutrients for the characteristics of low microbial activity, slow organic matter mineralization rate, and low nutrient availability in the existing cold-leaching fields An Important Way to Develop the Yield Potential of Middle and Low Yield Fields

Method used

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  • Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora
  • Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora
  • Psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and preparation method of psychrophilic microbial flora

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Experimental program
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Embodiment 1

[0030] (1) Enrichment culture of strain seed liquid: inoculate Pseudoalteromonas CCTCC M2010239, Psychrobacter sp ACCC11832, and yeast (Candida santamariae CGMCC No2959) into corresponding enrichment medium respectively , the number of strains reached the number of effective viable bacteria ≥ 2.0×10 under the constant temperature shaking culture for 4 days at the respective optimum culture temperature 9 CFU / mL.

[0031]Among them, the enrichment culture of Pseudoalteromonas is Zobell2216E medium (1000mL): tryptone 5g, yeast powder 1g, prepared with filtered aged seawater and tap water to 1000mL, filtered aged seawater:tap water=2:1 (volume ratio). The optimal culture temperature of Pseudoalteromonas is 6℃.

[0032] The enrichment culture of psychrophilic bacilli was YPD medium (1000mL): tryptone 5.0g, yeast extract powder 2.5g, glucose 1.0g, skimmed milk powder 1.0g, prepared to 1000mL with sterile water, pH value 7.0±0.1. The optimum temperature for cultivating psychrophili...

Embodiment 2

[0040] (1) Enrichment culture of strain seed liquid: Psychrophilic vibrio (Psychrophicvibrto2693, purchased from the Institute of Microbiology, Free University of Brussels) and Pseudoalteromonas CCTCC M2010239 were inoculated in the corresponding enrichment medium respectively, Under the respective optimal culture temperature, constant temperature shaking culture for 3 days, the number of bacteria reaches the number of effective viable bacteria ≥ 2.0×10 9 CFU / mL.

[0041] The enrichment culture of psychrophilic Vibrio was in marine inorganic medium (this medium was purchased from ixfcoLabolatories). The optimum culture temperature for psychrophilic Vibrio is 6°C.

[0042] Pseudoalteromonas enrichment culture is Zobell2216E medium (1000mL): tryptone 5g, yeast powder 1g, mixed with filtered aged seawater and tap water to make up to 1000mL, filtered aged seawater:tap water=2:1. The optimal culture temperature of Pseudoalteromonas is 6℃.

[0043] (2) Enrichment culture of psych...

Embodiment 3

[0047] (1) Enrichment culture of strain seed solution: Psychrophilic vibrio (Psychrophicvibrto2693, purchased from Institute of Microbiology, Free University of Brussels), Psychrobacter sp ACCC11832, Pseudomonas CGMCC NO1.39 , Pseudoalteromonas CCTCC M2010239, yeast (Candida santamariae CGMCC No2959), and Acinetobacter ATCC15309 were respectively inoculated in the corresponding enrichment medium, and cultured at constant temperature and shaking for 5 days at their respective optimum culture temperatures The number of bacteria reaches the number of effective viable bacteria ≥ 2.0×10 9 CFU / mL.

[0048] Psychrophilic Vibrio was enriched in marine inorganic medium (this medium was purchased from ixfcoLabolatories). The optimum culture temperature for psychrophilic Vibrio is 6°C.

[0049] The enrichment culture of psychrophilic bacteria was YPD medium (1000mL): 5.0g tryptone, 2.5g yeast extract powder, 1.0g glucose, 1.0g skimmed milk powder, distilled water to 1000mL, pH value 7....

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Abstract

The invention belongs to the technical field of microorganisms, and relates to a psychrophilic microbial flora activated by cold waterlogged paddy field nutrients and a preparation method of the psychrophilic microbial flora. The preparation method of the psychrophilic microbial flora activated by a cold waterlogged paddy field comprises the steps of mixing and inoculating more than two psychrophilic microbial strain culture liquid into nutrient nutrient broth, cultivating at constant temperature of 12-15 DEG C until the quantity of the strains achieves more than 1.0*10<10>CFU / mL to obtain the psychrophilic microbial flora; evenly adding 3-8% of psychrophilic microbial flora to an attachment carrier of the psychrophilic microbial flora according to the weight percent; and cultivating at constant temperature of 10-15 DEG C until the quantity of the strains achieves the condition that the quantity of effective living bacteria is more than 1.0*10<9> CFU / mL, so as to obtain the psychrophilic microbial flora activated by cold waterlogged paddy field nutrients. The psychrophilic microbial flora prepared by the method effectively prevents the phenomena of stifle of rice plant and runt seedling of paddy of the cold waterlogged paddy field, and promotes the crop to easily grow and rapidly develop, reduces the dosage of a chemical fertilizer, and benefits for conservation of cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a psychrophilic microbial flora activated by cold soaking field nutrients and a preparation method thereof. Background technique [0002] The cold soaking field is a low-yield field caused by long-term water immersion, muddy soil and strong reduction. There are about 3.46 million hm across the country 2 , accounting for 15.07% of the country's paddy field area and 44.2% of the low-yielding paddy field area. It is mainly distributed in the mountainous valleys and hilly low-lying areas of the southern provinces of my country. It is one of the main types of low-yielding rice fields in the southern rice areas. The rice yield per hectare is generally less than half of the average yield per hectare of rice fields. Statistics from the second soil census in Guangdong Province showed that there were 623,000 hectares of low-yielding fields in the province at that time 2 , t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C09K17/00C12R1/15C12R1/145C09K101/00
Inventor 徐培智解开治杨少海黄旭顾文杰逢玉万蒋瑞萍李苹黄巧义卢钰升付弘婷易琼张发宝唐拴虎陈建生刘春明
Owner GUANGZHOU XINNONGKE FERTILIZER SCI & TECH
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