Liquid phase isoelectric focusing electrophoresis system

Abstract

The invention relates to a liquid phase isoelectric focusing electrophoresis system which is characterized in that a conductive layer and an insulation layer are sequentially arranged on a bottom plate of a separating cavity of an original separating device so as to artificially form a stress capacitor, so that the capacitance effect in the separating cavity is reduced, and the anti-coupling capability of the system is improved; a plurality of cross beams which are parallel at uniform intervals are arranged above the separating cavity of the system and are fixed on a top plate of the separating cavity, a pressure rod with a pressure head is arranged on each end of each cross beam, and therefore, when each pressure rod bears a downward tensile force, pressures borne by all parts of the top plate of the separating cavity can be balanced; and a control unit is additionally arranged in an original power supply device, the voltage value, the current value and the power state output by a voltage pre-stabilizing unit in the power supply device are adaptively regulated, and the voltage, the current and the power of the power supply device are controlled to be constantly output through error feedback, and therefore, the stability of power supply output is further improved and the pH gradient of a buffer solution in the electrophoresis system is stable.

Description

technical field [0001] The invention relates to a liquid isoelectric focusing electrophoresis system. Background technique [0002] In recent years, liquid phase electrofocus electrophoresis technology has injected new vitality into the separation and analysis of biomacromolecules. Since the separation process of isoelectric focusing electrophoresis is basically completed in the liquid phase, continuous liquid isoelectric focusing electrophoresis can ensure that protein loss is minimized. In addition, liquid isoelectric focusing electrophoresis has a large separation capacity for protein concentration ranges. According to theoretical calculations, protein concentrations from 0.05mg / ml to 5mg / ml can be effectively separated. However, the existing liquid isoelectrofocus electrophoresis system has the following problems when it is working: [0003] 1. Since the separation chamber is in the "floating" state of high-voltage operation, the metal parts in the separation device, s...

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Problems solved by technology

Since the substrate of the separation device is an insulator, a random capacitive effect will be formed on both sides of the substrate, which will have an adverse effect on the stable operation of the liquid phase electrofocus electrophoresis system. , due to the capacitive effect, some samples will settle on the upper surface of the substrate, thus reducing the sample yield of the liquid isoelectric focusing electrophoresis system; on the other hand, the capacitive effect will also affect the buffer in the separation chamber, In turn, it affects the stability of the electric field formed by the positive and negative electrodes

[0004] 2. Since the formation of the ultra-thin cavity of the electrophoresis instrument depends on the gasket, but in the ultra-thin cavity, no metal components other than the electrodes are allowed to exist, so the gasket can only be an organic gasket with strong inertness.

However, in the above two methods, the continuous injection of buffer and the uninterrupted loading of samples will cause the conductance at the entrance of the separation electric field to be unstable, thus affecting the separation accuracy of electrophoresis.

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