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Intestine immunomodulator

An immunomodulatory and intestinal technology, applied in the field of intestinal immunomodulators, can solve the problems of aggravation of symptoms and insufficient mucosal protection, and achieve the effect of preventing infection

Active Publication Date: 2013-06-26
NIIGATA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, there are problems such as insufficient protection of mucous membranes, or insufficient suppression of aggravation of symptoms when the protected mucous membranes are mildly infected.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Lactobacillus paracasei K71 was inoculated into the MRS medium, and cultured at 37° C. for 20 hours. Lactic acid cells were recovered by centrifugation, washed twice with distilled water, dextrin equal to the weight of dry cells was added, and heat-sterilized at 80° C. for 30 minutes. The dead cell powder of Lactobacillus paracasei K71 (hereinafter abbreviated as "K71 powder") was obtained after freeze-drying.

[0048] BALB / c female mice (5 weeks old, purchased from Charles River Company) were acclimatized and raised in a normal environment at 22°C for one week, and then divided into two groups, with 5 mice in each group. Suspend 20 mg of K71 powder in 1 mL of sterilized water, and administer 50 μL of the suspension to one of the groups orally once a day (1 mg-K71 powder / mouse / day) (K71 administration group). Similarly, another group was given 50 μL of sterilized water once a day (control group). In the fifth week of feeding (from the night of the 34th day to the morn...

Embodiment 2

[0051] As in Example 1, 1 mg / mouse / day of K71 powder was repeatedly orally administered to BALB / c female mice (6 mice in each group), and the content of SIgA in feces was measured once a week. As a result, such as figure 2 It is shown that during the whole test period, the SIgA content of the K71 administration group showed a higher value compared with the control group, and the difference was statistically significant in the 2nd, 4th and 6th week (P<0.05).

Embodiment 3

[0053] Same as in Example 1, BALB / c female mice (6 in each group) were given K71 powder or sterilized water every day. The splenocytes were collected after 5 weeks of feeding. Splenocytes (1.25×10 6 cells / ml) and YAC-1 cells (1×10 5 cells / ml) at a ratio of 12.5:1 (spleen cells:YAC-1 cells), in RPMI-1640 medium (96-well culture plate) containing 1% fetal bovine serum, at 37°C, 5% CO 2 conditions for 4 hours. By investigating the activity of lactate dehydrogenase (LDH) released as the YAC-1 cells were destroyed, the cell killing activity of the collected spleen cells was determined. Specifically, 50 μL of the co-culture supernatant of splenocytes and YAC-1 cells was transferred to a new 96-well culture plate, and the LDH enzyme activity was measured using Roche Cytotoxicity Detection Kit (LDH).

[0054] The result is as image 3 As shown, compared with the control group, the cell killing activity of splenocytes in the K71-administered group showed a higher value, which was ...

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Abstract

The purpose of the present invention is to provide a better intestine immunomodulator. The intestine immunomodulator of the present invention comprises bacterial cells or a bacterial component of a Lactobacillus paracasei K71 strain having an international deposit No.: FERM BP-11098 as an active ingredient. Preferably, the intestine immunomodulator is used to facilitate production of secretory immunoglobulin A or to activate natural killer cells.

Description

technical field [0001] The present invention relates to an intestinal immunomodulator, and more specifically relates to an intestinal immunomodulator containing specific lactic acid bacteria cells or bacterial cell components as active ingredients. Background technique [0002] Secretory immunoglobulin A (SIgA) exists on the mucous membranes of the mouth, nostrils, respiratory organs, digestive tract, etc., and is an immune substance that constitutes the core of mucosal immunity. SIgA plays an important role in protecting mucous membranes from exposure to pathogens and allergens through outside air and food. That is, SIgA binds to bacteria and viruses to aggregate them, and at the same time binds to toxins and enzymes derived from bacteria to detoxify them, thereby protecting mucous membranes. The secretion of SIgA is controlled by the brain, endocrine system and autonomic nervous system, and the secretion of SIgA is lower in the young and the elderly than in adults. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P37/02A61P43/00A61K35/747A61P31/04
CPCA23L33/135A61K35/747A61P31/04A61P37/02A61P43/00A61K35/74
Inventor 原崇樋口裕树藤井干夫
Owner NIIGATA UNIVERSITY
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