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Method for controlling the production of low molecular weight heparin

A low molecular weight heparin, molecular weight technology, applied in the field of controlled production of low molecular weight heparin or ultra-low molecular weight heparin, can solve problems such as limiting the application of heparin

Active Publication Date: 2016-06-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the anticoagulant activity of heparin, a large amount of heparin will cause side effects such as bleeding and induction of thrombocytopenia, which greatly limits the clinical application of heparin

Method used

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  • Method for controlling the production of low molecular weight heparin
  • Method for controlling the production of low molecular weight heparin
  • Method for controlling the production of low molecular weight heparin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] The (ultra) low molecular weight heparin product obtained in Example 1 adopts high performance exclusion chromatography to measure its average molecular weight (including the weight average molecular weight M w and number average molecular weight M n ) and distribution. The gel chromatography column used is TSKGelG3000SW, 30mm×750mm, Tosoh Co., Japan. The HPLC system used includes computer control system, peristaltic pump (LC-10ATvp), autosampler (SIL-10ADvp), ultraviolet detector (SPD-M10Avp), differential detector (RID-10A). Wherein the ultraviolet detector and the differential detector are connected in series, and the differential detector is connected behind the ultraviolet detector. The mobile phase used is pH5.0, containing 2.84% Na by mass percentage 2 SO 4 buffer. The column temperature is 35°C, the flow rate is 0.5mL / min, and the detection wavelength of the ultraviolet detector is 235nm.

[0113] (Ultra) low molecular weight heparin molecular weight stand...

Embodiment 2

[0141] Example 2 Comparison of heparinase I and heparinase III degrading heparin

[0142] Add equal amounts of heparinase I and heparinase III to the heparin solution, degrade completely at 30°C, and react until A 235 No change occurs. The product was then passed through a membrane, precipitated and evaporated to dryness under reduced pressure. The products after evaporation to dryness were subjected to molecular weight identification and anti-Xa, IIa factor activity detection, and the results are shown in Table 2. Among them, the 1# sample is the product obtained by the degradation of heparinase I, with low molecular weight and low anti-Xa and anti-IIa activities, indicating that heparanase I can cut the anticoagulant active center structure of heparin. With the prolongation of cutting time, Most of the active centers are destroyed; 2# sample is the degradation product of heparanase III, which has a larger molecular weight than 1# sample, and the important thing is that t...

Embodiment 3

[0146] Example 3 Comparison of heparinase III and heparanase III, I combined use to degrade heparin

[0147] In order to test the differences in the action sites of heparanase I and heparanase III, the following experiments were designed. First use heparanase III to degrade heparin, degrade it completely at 30°C, and react until A 235 No change occurs, then take out half of the solution, then add heparanase I to it for cleavage, keep the other half of the solution as a control, and finally evaporate the two samples to dryness under reduced pressure. The products after evaporation to dryness were subjected to molecular weight identification and anti-Xa, IIa factor activity detection, and the results are shown in Table 3. Among them, the 2# sample is the product obtained by the degradation of heparanase III (see Example 2), with a molecular weight of 8299; the 3# sample is firstly degraded completely by using heparanase III under the same conditions as in Example 5 at 30°C ,...

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Abstract

This article discloses a production method of low molecular weight heparin or ultra-low molecular weight heparin. This method utilizes two or more heparinase enzymes selected from heparinase I, II and III to degrade heparin to produce low molecular weight heparin or ultra-low molecular weight heparin.

Description

technical field [0001] The invention relates to a method for controlling the production of low molecular weight heparin or ultra-low molecular weight heparin. The method utilizes two or more heparinases selected from heparinase I, II and III to degrade heparin to produce low molecular weight heparin or ultra-low molecular weight heparin. Molecular weight heparin method. Background technique [0002] Heparin is a mucopolysaccharide formed by hexuronic acid (L-iduronic acid, D-glucuronic acid) and D-glucosamine sulfate by alternating 1→4 glycosidic bonds, and has a linear structure of repeating units of six or eight sugars. Chain structure, its molecular weight is between 3000-37000Da, it is used as anticoagulant reagent and antithrombotic reagent in medicine. In addition, heparin also has various biological functions such as anti-inflammation, anti-allergy, anti-virus, anti-cancer, and blood lipid regulation. However, because heparin has anticoagulant activity, a large amou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/00C12N9/88
Inventor 邢新会李晔吴敬君张翀冯权毕鲜荣
Owner TSINGHUA UNIV
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