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Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof

A technology of Phytophthora capsicum and Pepper blight, applied in the fields of application, bacteria, biocide, etc., can solve the problem of high spontaneous mutation frequency of drug resistance, achieve remarkable control effect, increase economic income, and save expenses

Inactive Publication Date: 2015-01-28
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies have shown that some pathogenic bacteria targets have a high frequency of spontaneous mutations that are resistant to such agents, and pathogenic bacteria are prone to form drug-resistant populations under the high selection pressure of agents.

Method used

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  • Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof
  • Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof
  • Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the separation and identification of biocontrol bacteria G1

[0029] 1. Isolation and identification of strains

[0030] (1) Isolation of strains

[0031] The pepper plants collected from the Science and Technology Innovation Park of Shandong Agricultural University were rinsed with tap water, divided into three parts: root, stem, and leaf, and then rinsed twice with sterile water, and the surface moisture was blotted dry with sterile filter paper. Under sterile conditions, soak in 75% ethanol for 1 min, rinse with sterile water for 3 times, transfer to 3% sodium hypochlorite disinfectant for 5 min, rinse with sterile water for 3 times, and dry the surface with sterile filter paper. Place the surface-sterilized roots, stems and leaves in a sterilized mortar, cut them into pieces, add an appropriate amount of sterile water, grind them, and then dilute them to 10 -1 、10 -2 、10 -3 Three gradients, each taking 10 -2 、10 -3 Transfer 200 μL each of the dil...

Embodiment 2

[0055] Embodiment 2, the application of bacterium G1 in the prevention and treatment of capsicum blight

[0056] 1. Preparation of G1 bacterial agent

[0057] (1) Preparation of seed solution:

[0058] The Paenibacillus polymyxa G1CGMCC No.6762 isolated and preserved in Example 1 was inoculated on LA medium containing a concentration of 500 μg / mL streptomycin sulfate (Ster) resistance marker, and after being activated at 28°C, it was transferred into a 100 mL of LB culture solution was placed in a 250 mL Erlenmeyer flask at 28° C. and 180 rpm for 24 hours with shaking to obtain G1 seed solution.

[0059] LA medium: peptone 10g, yeast powder 5g, NaCl 5g, agar powder 17g, distilled water 1000mL, pH 7.0; LB medium: no agar powder, other ingredients are the same as LA medium.

[0060] (2) Preparation of bacterial agent:

[0061] When the G1 seed solution is cultivated to an OD value of 0.5, add the G1 seed solution to the LB culture medium at a volume ratio of 1:100, shake and ...

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Abstract

The invention discloses a bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof. The invention provides a paenibacillus polymyxa strain G1 the preservation No. of which is CGMCC No. 6762. The invention also provides a method for preparing a G1 bacteria agent, which comprises the following steps: fermenting the paenibacillus polymyxa strain G1, and collecting fermented products so as to obtain the G1 bacteria agent. Experiments prove that aiming at the current situation that no bio-control agent which achieves a good effect on preventing pepper phytophthora blight is provided at present; and the invention provides a single bacterium for preventing pepper phytophthora blights and a preparation thereof, which are specially applied to the prevention and control of pepper phytophthora blights. The bacteria agent has a good prevention and control effect on important soil-borne diseases, and the control effect on greenhouse pepper phytophthora blights reaches 71.18%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biocontrol strain G1 for preventing and treating pepper blight and application thereof. Background technique [0002] Pepper blight is an oomycete disease caused by Phytophthora capsici. Phytophthora belongs to Oomycota, Peronomyces, Pythiumceae, and Phytophthora. Phytophthora survives in soil not in one form, but in different forms, namely mycelium, sporangia, chlamydospores and oospores. The disease can be transmitted through rainwater, irrigation water, and agricultural implements, and is a highly harmful soil-borne disease. With the large-scale planting of peppers in my country, pepper blight also occurs in large areas in open fields, protected areas, and greenhouses. Because the disease has a short onset period and rapid prevalence, it often leads to the death of pepper plants in patches and brings serious economic losses to the pepper industry. Generally, the rate of disea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
Inventor 高克祥刘晓光赵影何邦令王庆华张修国
Owner SHANDONG AGRICULTURAL UNIVERSITY
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