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A culture method for improving the degradation performance of Phanerochaete chrysosporium

A technology of Phanerochaete chrysosporium and its cultivation method, which is applied in the cultivation field of improving the degradation performance of Phanerochaete chrysosporium, can solve the problems urgently needed to be developed, and achieve the improvement of lignin peroxidase and catalytic activity, large specific surface area, significant effect

Inactive Publication Date: 2016-01-06
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have their advantages and disadvantages, but in order to further improve the degradation performance of Phanerochaete chrysosporium and expand its application research, new regulation methods still need to be developed urgently

Method used

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  • A culture method for improving the degradation performance of Phanerochaete chrysosporium
  • A culture method for improving the degradation performance of Phanerochaete chrysosporium
  • A culture method for improving the degradation performance of Phanerochaete chrysosporium

Examples

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Effect test

Embodiment 1

[0026] A kind of culture method that improves Phanerochaete chrysosporium degradation performance, it comprises the steps:

[0027] (1) Strain activation: Phanerochaete chrysosporium was inoculated on the plate medium for activation culture, the culture temperature was 37°C, and after 2-3 days of culture, it was transferred to room temperature for culture until the mycelia covered the plate;

[0028] (2) Adding inorganic nanoparticles: Based on 1L liquid medium, add 20.0g of SiO 2 Nanoparticles, the particle diameter of the nanoparticles is 20nm, so as not to add the SiO 2 The medium of nanoparticles is used as a comparative example;

[0029] (3) Cultivation of bacteria: Prepare the activated strain obtained in step (1) into a spore suspension, and inoculate it into the SiO 2 Nanoparticle-based liquid medium supplemented with SiO per liter 2 The spore inoculum in the liquid medium of nanoparticles was 2.0×10 6 ~3.0×10 6 one, cultured on a shaking table at 30° C., the rota...

Embodiment 2

[0038]The cultivation method for improving the degradation performance of Phanerochaete chrysosporium in this example is roughly the same as in Example 1, except that: 1) Based on 1L liquid medium, add 0.1g of SiO 2 Nanoparticles, the particle size of the nanoparticles is 350nm; 2) The conditions of the fermentation culture are: culture on a shaking table at 32° C., the speed of the shaking table is 145 rpm, and cultivate for 5 to 7 days.

[0039] Carry out the test of dye decolorization rate and adsorption rate according to the method identical with embodiment 1. Within 6 hours, the decolorization rate of the dye increased from 21% to 70%, an increase of about 3.5 times; within 1 hour, the adsorption rate of the dye increased from 15% to 70%, an increase of about 4.5 times.

Embodiment 3

[0041] The cultivation method for improving the degradation performance of Phanerochaete chrysosporium in this example is roughly the same as in Example 1, except that: 1) Based on 1L of liquid medium, 3.0g of SiO 2 Nanoparticles, the particle size of the nanoparticles is 350nm; 2) The conditions of the fermentation culture are: culture on a shaking table at 33° C., the speed of the shaking table is 140 rpm, and cultivate for 5 to 7 days.

[0042] Carry out the test of dye decolorization rate and adsorption rate according to the method identical with embodiment 1. Within 6 hours, the dye decolorization rate increased from 21% to 49%, an increase of about 2.3 times; within 1 hour, the dye adsorption rate increased from 15% to 48%, an increase of about 3.3 times.

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Abstract

The invention relates to a cultivation method for improving the degradation performance of Phanerochaete chrysosporium, belonging to the field of bioengineering. In the present invention, the silicon-based nanoparticles are added to the Phanerochaete chrysosporium liquid medium for fermentation and cultivation, and through the interaction between the silicon-based nanoparticles, spores and mycelia, the nanoparticles enter the interior of the mycelium ball to obtain spherical yellow Phanerochaete sporogenes mycelial group, the mycelial group has a better application effect in dye degradation.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a cultivation method for improving the degradation performance of Phanerochaete chrysosporium. Background technique [0002] Phanerochaete chrysosporium ( Phanerochaetechrysosporium ) is a class of filamentous basidiomycete white-rot fungi that contain an excellent lignin decomposition system. This excellent lignin decomposition system consists of a series of extracellular peroxidases, typical enzymes such as lignin peroxidase and manganese peroxidase. These extracellular peroxidases are highly non-specific in the oxidation of substrates, and can degrade a variety of organic substances, including natural polymers, reducing compounds, oxidizing compounds, and toxic compounds. Therefore, Phanerochaete chrysosporium has a wide application value in many biotechnology fields, such as the treatment of hazardous waste, the bioremediation of polluted land, the preparation of biofuel, biopulp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 苏宝连李其昌李昱谢浩
Owner WUHAN UNIV OF TECH
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