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Method for detecting L-cysteine concentration by applying of circular dichroism

A technology of cysteine ​​and circular second spectroscopy, applied in the field of analytical chemistry, to achieve the effect of improving sensitivity and simplifying reaction conditions

Active Publication Date: 2014-11-26
JIANGNAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, the detection method for constructing L-cysteine ​​by this assembly system combined with the classical adsorption principle of L-cysteine ​​is still blank.

Method used

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  • Method for detecting L-cysteine concentration by applying of circular dichroism
  • Method for detecting L-cysteine concentration by applying of circular dichroism
  • Method for detecting L-cysteine concentration by applying of circular dichroism

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Synthesis of 25nm gold nanoparticles

[0031] 25nm gold nanoparticles were synthesized by one-step reduction of chloroauric acid with three sodium citric acid: add 47.5mL ultrapure water to a clean three-neck flask, add 0.8mL 0.4% chloroauric acid solution, stir and heat to boiling, after 7-8min Add 1mL of 1% trisodium citrate solution, after the solution turns from colorless to red, stop heating and continue stirring for 30min; transmission electron microscope shows that the average particle size is 25nm;

[0032] (2) Assembly of gold nanoparticles

[0033] Take 1000 μL of 25nm gold nanoparticles prepared in step (1), centrifuge at 8000 rpm for 10 minutes, resuspend into the same volume of 0.01 M pH6.0 phosphate buffer, add 100 μL of 50nM L-cysteine ​​solution to react for 30 minutes, Assembly to form gold nanoparticle dimers;

[0034] Choice of buffer pH

[0035] In order to improve the sensitivity and stability of this method, it is necessary to select the pH ...

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Abstract

The invention discloses a method for detecting L-cysteine concentration by applying of a circular dichrorism, and belongs to the technical field of analytical chemistry. The L-cysteine and a reagent are mixed according to a certain volume ratio to generate self-assembly and form gold nanoparticle dimer, reactant is placed on a circular dichroism analyzer, signal strength at 525nm position of a main wave is detected, and therefore the concentration of the L-cysteine is measured. Compared with a traditional instrument, under a liquid environment, AuNPs and the L-cysteine are utilized to react to form the gold nanoparticle dimer, the chirality of the L-cysteine can be enlarged through the form of a binary assembly, and a CD signal is produced by the gold nanoparticle diamer in a visible region. The contents of the L-cysteine are measured by a manner that the CD signal intensity under the liquid environment is measured through the circular dichrorism. The method only can be carried out under the liquid environment, and a step of washing is not needed. Only one step of reaction is needed, reaction conditions are simplified, and sensitivity of detection is improved.

Description

technical field [0001] A method for detecting the concentration of L-cysteine ​​by using circular dichroic spectroscopy, which relates to a method for measuring the concentration of L-cysteine ​​by combining gold nanoparticle self-assembly technology and plasma chiral technology, which belongs to analytical chemistry technology field. Background technique [0002] Currently commonly used cysteine ​​detection methods are based on physical or chemical methods, such as inductively coupled plasma emission spectrometry, flow injection atomic absorption spectrophotometer, inductively coupled plasma mass spectrometry, polarography and voltammetric electrodes, Atomic fluorescence spectroscopy, atomic furnace absorption spectroscopy. The biggest advantage of the above-mentioned main instrument detection methods is that they are accurate, but these traditional methods require complicated pre-treatment, long analysis time, high labor intensity and high cost, and can only be performed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/19
Inventor 胥传来王利兵许宙徐丽广马伟李灼坤
Owner JIANGNAN UNIV
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