Induced proliferation method for glehnia littoralis hairy roots
A technology of Coral vegetables and hairy roots is applied in the field of the induction and proliferation of Coral vegetables hairy roots.
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[0036] 1. Occurrence of callus on leaves of coral dish
[0037] 1. Experimental materials
[0038] The plant material is selected from coral vegetable leaves free of diseases and insect pests.
[0039] The sucrose concentration in MS (Murashige and Skoog) medium was 30g / L, and the agar content was 8g / L.
[0040] The induction medium of callus is MS+2,4-D0.15mg / L+6-BA1.8mg / L+NAA0.2mg / L.
[0041]2. Biochemical reagents and main instruments
[0042] The components used in the MS medium, as well as alcohol, formaldehyde, glacial acetic acid, xylene and other reagents are domestic analytical reagents. Cytokinin 6-BA, auxin NAA, and 2,4-D are all chemically pure products of Shanghai Bioengineering Company.
[0043] Commonly used instruments for tissue culture: aseptic operating table, portable pressure steam sterilizer, HPG-280B light incubator, etc.
[0044] 3. Experimental method
[0045] 3.1 Treatment of explants
[0046] Take some coral dish leaves, wash off the dust on t...
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