Method for preparation of flavone by culturing radix puerariae cells
A cell and flavonoid technology, applied in the field of cell engineering, can solve the problems of incomplete extraction, unstable flavonoid yield, loss of flavonoid components, etc.
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Embodiment 1
[0034] Embodiment 1: Preparation of flavonoids by culturing kudzu root cells
[0035] 1. Prepare medium (mass percentage concentration)
[0036] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;
[0037] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;
[0038] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;
[0039] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;
[0040] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;
[0041] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water; ...
Embodiment 2
[0049] Embodiment 2: Preparation of flavonoids by culturing kudzu root cells
[0050] 1. Prepare medium (mass percentage concentration)
[0051] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;
[0052] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;
[0053] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;
[0054] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;
[0055] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;
[0056] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water; ...
Embodiment 3
[0064] Embodiment 3: Preparation of flavonoids by culturing kudzu root cells
[0065] 1. Prepare medium (mass percentage concentration)
[0066] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;
[0067] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;
[0068] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;
[0069] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;
[0070] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;
[0071] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water;
...
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