Preparation method of banana extract for fermented cigarette
An extract and fermented technology, which is applied in the field of preparation of fermented banana extract for tobacco, can solve the problems of inability to produce new flavors for tobacco and difficulty in filtration, and achieve the goal of accelerating filtration speed, increasing categories, and soft and delicate smoke Effect
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Embodiment 1
[0028] A preparation method of fermented tobacco banana extract, comprising the following steps:
[0029] 1) Culture medium preparation
[0030] a. Raw material pretreatment: beat banana pulp to make banana puree, mix it with water according to the weight ratio of 1:5, vibrate to obtain banana aqueous solution;
[0031] b. Add sodium chloride: add 0.02% sodium chloride by weight to the prepared banana aqueous solution, and sterilize at 115°C for 15 minutes to obtain a culture medium;
[0032] 2) Vaccination
[0033] a. Preparation of composite bacteria preparation: mix Rhizopus jijiji and Mucor racemosa at a mass ratio of 2:1, and add 5 times the mass of water to prepare the bacterial solution.
[0034] b. Inoculation: under sterile conditions, inoculate the compound bacterial preparation in the culture medium, the inoculum amount is 0.5%;
[0035] 3) fermentation
[0036] Ferment for 5 days at a pH value of 7, a stirring speed of 50 rpm, an aeration rate of 0.2 VVM, and a...
Embodiment 2
[0042] A preparation method of fermented tobacco banana extract, comprising the following steps:
[0043] 1) Culture medium preparation
[0044] a. Raw material pretreatment: beat banana pulp to make banana puree, mix it with water at a weight ratio of 1:10, and vibrate to obtain a banana aqueous solution;
[0045] b. Add sodium chloride: add 0.005% sodium chloride by weight to the prepared banana aqueous solution, and sterilize at 115°C for 20 minutes to obtain a culture medium;
[0046] 2) Vaccination
[0047] a. Preparation of composite bacteria preparation: mix Rhizopus jijiji and Mucor racemosa in a mass ratio of 1:1, and add 8 times the mass of water to prepare the bacterial solution.
[0048] b. Inoculation: under aseptic conditions, inoculate the compound bacterial preparation in the culture medium, the inoculum amount is 1.0%;
[0049] 3) fermentation
[0050] Ferment for 8 days at a pH value of 5, a stirring speed of 100 rpm, an aeration rate of 0.5 VVM, and a fe...
Embodiment 3
[0056] A preparation method of fermented tobacco banana extract, comprising the following steps:
[0057] 1) Culture medium preparation
[0058] a. Raw material pretreatment: beat banana pulp to make banana puree, mix it with water according to the weight ratio of 1:8, vibrate to obtain banana aqueous solution;
[0059] b. Add sodium chloride: add 0.01% sodium chloride by weight to the prepared banana aqueous solution, and sterilize at 115°C for 25 minutes to obtain a culture medium;
[0060] 2) Vaccination
[0061] a. Preparation of composite bacteria preparation: mix Rhizopus jiji with Mucor racemosa at a mass ratio of 4:1, and add 8 times the mass of water to prepare the bacterial solution.
[0062] b. Inoculation: under aseptic conditions, inoculate the compound bacterial preparation in the culture medium, the inoculum amount is 1.0%;
[0063] 3) fermentation
[0064] Ferment for 5 days at a pH value of 8, a stirring speed of 80 rpm, an aeration rate of 0.8 VVM, and a ...
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