Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof
A technology of Trichosporium chinensis and proteoglycan, which is applied in the field of Trichospora chinensis proteoglycan and its preparation, can solve the problems that do not involve the identification of polysaccharide activity or advanced structure, and achieve low cost, clear structure and efficient development. Effect
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Embodiment 1
[0044] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculum amount of 6% (weight percentage), and culture it statically for 40 days at 18°C to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 20%, peptone 5%, cicada pupa powder 10%, KH 2 PO 4 1.0%, MgSO 4 .7H 2 O 0.5%, the balance is sterile water.
[0045] (2) Alcohol precipitation: Concentrate the Mortierella sinensis fermentation liquid in step (1) to obtain a concentrated liquid, add 4 times the volume of the concentrated liquid, and the volume percentage concentration is 95% ethanol aqueous solution, stir and mix, place in 4 Precipitate overnight in the refrigerator at ℃, then centrifuge at 6000rpm at 4℃ for 25min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis once;
[0046] (3) Deproteinization: dissolving the crude polysaccharide of Mortierella...
Embodiment 2
[0052] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculum amount of 5% (weight percentage), and culture it statically for 50 days at 15°C to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 15%, peptone 3%, cicada pupa powder 6%, KH 2 P O 4 0.6%, MgSO 4 .7H 2 O 0.1%, the balance is sterile water.
[0053] (2) Alcohol precipitation: Concentrate the fermented liquid of Mortierella sinensis in step (1) to obtain a concentrated liquid, add 5 times the volume of the concentrated liquid, and the volume percentage concentration is 96% ethanol aqueous solution, stir and mix, place in 2 Precipitate overnight in the refrigerator at ℃, then centrifuge at 5000rpm at 2℃ for 30min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis;
[0054] (3) Deproteinization: dissolving the crude polysaccharide of Mortierella sinensi...
Embodiment 3
[0060] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculation amount of 10% (percentage by weight), and culture it statically for 30 days at 22°C to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 25%, peptone 7%, cicada pupa powder 12%, KH 2 PO 4 1.2%, MgSO 4 .7H 2 O 1%, the balance is sterile water.
[0061] (2) Alcohol precipitation: Concentrate the Mortierella sinensis fermentation liquid in step (1) to obtain a concentrated liquid, add 4 times the volume of the concentrated liquid, and the volume percentage concentration is 90% ethanol aqueous solution, stir and mix, place in 5 Precipitate overnight in the refrigerator at ℃, then centrifuge at 4000rpm at 5℃ for 30min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis once;
[0062] (3) Deproteinization: dissolving the crude polysaccharide of Mortiere...
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