Method for screening plants using bicarbonate radical ions efficiently by using photosynthetic carbon dioxide response curve

Abstract

The invention discloses a method for screening plants using bicarbonate radical ions efficiently by using a photosynthetic carbon dioxide response curve. The method includes the following steps: firstly, determining photosynthetic carbon dioxide response curves of studied plants and reference plants respectively by using a conventional method of a portable photosynthetic instrument; secondly, calculating water use rate of the studied plants and the reference plants under different carbon dioxide contents according to net photosynthetic rate and transpiration rate of the studied plants and the reference plants under different carbon dioxide contents in the photosynthetic carbon dioxide response curves determined by the portable photosynthetic instrument; thirdly, fitting plant water use rate data under carbon dioxide content lower than 320 mumol-mol<-1> into a linear equation in which the plant water use rate changes along with the carbon dioxide content; and obtaining gradient of the equation, namely leaf apparent water carbon use efficiency; and fourthly, screening the plants using the bicarbonate radical ions efficiently according to ratio of leaf apparent water carbon use efficiency of the studied plants to leaf apparent water carbon use efficiency of the reference plants.

Description

technical field [0001] The invention relates to the fields of afforestation and ecological management, in particular to a method for screening plants that utilize bicarbonate ions efficiently using the carbon dioxide response curve of photosynthesis. Background technique [0002] China's karst (karst) distribution area is about 1.3 million Km 2 , of which the rocky desertification area accounts for about 28.7%. "South rock (rocky desertification) and northern sand (desertification)" have become two major ecological environmental problems that restrict the sustainable development of the western region. The vulnerability of the karst environment is mainly manifested in the characteristics of exposed bedrock (rocky desertification), rock permeation and water leakage (karst drought), barren soil (low nutrition), high pH, ​​high bicarbonate and calcium and magnesium concentrations; Vulnerability seriously affects plant growth and development as well as carbon sequestration and ...

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Problems solved by technology

[0006] Although, at present, we cannot use instruments such as the Li-6400 portable photosynthesis instrument to directly measure the ability of plant leaves to utilize bicarbonate ions, and it is also difficult to directly use existing photosynthetic indicators to screen plants that efficiently utilize bicarbonate ions. However, Recently, through the study of the carbon dioxide response curve of plant photosynthesis, it was found that in the case of low concentration of carbon dioxide, some plant leaves are supplemented by water and carbon dioxide converted by bicarbonate ions under the action of carbonic anhydrase, which greatly reduces the utilization of leaves. The efficiency of carbon dioxide and water in the whole air chamber, by selecting reference plants, comparative studies can provide information for screening plants that efficiently utilize bicarbonate ions, and the present invention is realized based on such principles

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