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Corallococcus coralloides and application thereof

A kind of coralline coccus and drug technology, applied in the field of applied microbiology, can solve the problems of few model strains, difficult screening and cultivation, etc.

Active Publication Date: 2013-05-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As two major types of myxobacteria, the genus Solexis and Pseudomonas genus, more and more strains have been screened out. It is difficult to screen and cultivate, and there are very few model strains that have been screened and studied

Method used

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  • Corallococcus coralloides and application thereof
  • Corallococcus coralloides and application thereof
  • Corallococcus coralloides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1. Isolation and purification of bacterial strains

[0035] 1.1 After the sample is collected, let it dry naturally at room temperature (to reduce the pollution of mold and miscellaneous bacteria).

[0036] Soil samples were collected from Dongying, Shandong and air-dried naturally at room temperature.

[0037] 1.2 Induction of myxobacteria fruiting bodies

[0038] 1.2.1 Fecal particle induction plate method: Put about 20 grams of air-dried soil samples in a petri dish, half bury 3-4 sterilized rabbit feces on the surface of the soil samples, add 25 μg / mL ether sterilized put After soaking for 6 hours with cycloheximide (25mg / ml) or carbendazim (30mg / L), pour off the residual liquid, incubate at 30°C, and observe the formation of fruiting bodies on the feces ball after 48 hours.

[0039] 1.2.2 Yeast induction plate method: use WCX (penicillin penicillin final concentration 100mg / L) medium as the base medium, pour it into a sterile plate, draw a cross line w...

Embodiment 2

[0052] Embodiment 2. Identification of strains

[0053] 2.1 Morphological features ( image 3 )

[0054] The isolated and screened myxobacteria EGB were negative by Gram staining, and the morphology of the strain was observed under a 100× oil lens through crystal violet staining. It was found that the strain was rod-shaped, without flagella, and transparent at both ends , under the transmission electron microscope, there is a layer of mucus around the single bacteria; through the light microscope 10× observation, it is found that the strain has a colony effect during the growth process, and the single bacteria gather together to form fruiting bodies in the late growth period.

[0055] 2.2 Identification

[0056] Through the PCR amplification of the 16S rDNA of the strain, the 16S rDNA gene sequence of the strain was obtained, logged in NCBI (www.ncbi.nlm.nih.govPblastP), and the obtained sequencing results were compared with the known sequences in the database using BLAST s...

Embodiment 3

[0057] Embodiment 3. Study on Resistance of Corallococcus sp. EGB to Several Phytopathogenic Fungi

[0058] 3.1 Select a suitable medium (angie yeast 0.5%, CaCl 2 2H 2 O0.1%, potato 30%, glucose 2%, agar 1.5%) make strain EGB (CCTCC NO: M2012528) and several plant pathogenic fungi: cotton fusarium wilt, cucumber yellow wilt and rice smut grow without interfering with each other. First pick several kinds of plant pathogenic fungal hyphae to the center of the plate, after the pathogenic fungus grows strong hyphae, connect the isolated myxobacteria EGB (CCTCC NO: M2012528) on both sides, and culture at 30°C for 4-7 days , it was found that the surrounding mycelia of several selected plant pathogenic bacteria withered gradually, and their growth was obviously inhibited ( Figure 5 ).

[0059] 3.2 Inoculate the strain EGB (CCTCC NO: M2012528) into VY / 4 liquid medium (Angelica 0.5%, CaCl 2 2H 2 O0.1%pH7.0), cultured at 30°C for 3-4 days, centrifuged at 6000rpm for 20min, coll...

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Abstract

The invention belongs to the field of applied microbiology, and relates to Corallococcus coralloides and application thereof. The Corallococcus coralloides EGB is preserved in China Center for Type Culture Collection (CCTCC), wherein the preservation data is 17th, December, 2012 and the preservation number is CCTCC NO: M2012528. In the growing process of the strain, the strain has good inhibiting effect to growth of plant pathogenic fungi of paddy false smut, fusarium wilt of cotton and fusarium wilt of cucumber. The fermented supernate of the strain has remarkable splitting action to hypha of pathogenic fungi. Meanwhile, abundant secondary metabolites generated by the strain have potential research value.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and relates to a strain of coral coccus and its application in the prevention and treatment of pathogenic fungi. Background technique [0002] Rice is the main food crop in some production areas in my country and even in the world. Rice false smut is a fungal disease that commonly occurs in the late stage of rice production in major rice-growing areas in my country. %, bringing huge economic losses to the vast rice-producing areas. In recent years, due to changes in climatic conditions and unreasonable fertilization and cultivation, the damage of rice false smut has become increasingly serious. [0003] Cotton is an important economic crop in my country, and it is grown in most provinces. According to reports, there are more than 120 kinds of cotton diseases in the world, and more than 40 kinds have been found in my country, of which 15 are seriously damaged. Blight, blight, and anthracnose ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 崔中利李周坤黄彦
Owner NANJING AGRICULTURAL UNIVERSITY
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