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Reagent composition for nucleic acid chromatography or immunochromatography, method for measurement by nucleic acid chromatography or immunochromatography, and kit for measurement by nucleic acid chromatography or immunochromatography

A technology of immunochromatography and composition, which is applied in the fields of reagent composition for nucleic acid or immunochromatography, nucleic acid or immunochromatography determination, and nucleic acid or immunochromatography assay kits, which can solve the problem that additives have not sufficiently achieved non-specific reactions Inhibition or sensitivity chromatographic carrier expansion, non-specificity, lack of sensitivity, etc., to achieve accurate and rapid judgment, promote specific reactions, and improve dispersion capabilities

Inactive Publication Date: 2013-05-08
TANAKA PRECIOUS METAL IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are problems in that if reactants such as antibodies and nucleic acids are increased, non-specific reactions are likely to occur, and in addition, sufficient sensitivity cannot be obtained if a small amount is used.
[0005] However, none of the combinations of additives has sufficiently achieved the suppression of non-specific reactions, the improvement of sensitivity, and the expandability on the chromatographic support.

Method used

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  • Reagent composition for nucleic acid chromatography or immunochromatography, method for measurement by nucleic acid chromatography or immunochromatography, and kit for measurement by nucleic acid chromatography or immunochromatography
  • Reagent composition for nucleic acid chromatography or immunochromatography, method for measurement by nucleic acid chromatography or immunochromatography, and kit for measurement by nucleic acid chromatography or immunochromatography
  • Reagent composition for nucleic acid chromatography or immunochromatography, method for measurement by nucleic acid chromatography or immunochromatography, and kit for measurement by nucleic acid chromatography or immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～8 and comparative example 1～4

[0093] In the amount recorded in Table 1, 10% Tween20, 0.1M magnesium sulfate, dimethyl sulfoxide, 20% dextran sodium sulfate (weight average molecular weight: 500,000), and 2% of CE510 (JSR Corporation) and mixed. Further, 10% sodium azide was added as a preservative to make it 0.05%, and mixed to obtain a reagent composition.

[0094] [Table 1]

[0095]

Embodiment 9～14 and comparative example 5～9

[0097] Add 2% of a water-soluble polymer with a weight average molecular weight of 8,000 or more, 5mM of a divalent or trivalent metal salt, and 1% of a nonionic surfactant to ultrapure water of the type shown in Table 2 , 0.95% of a water-soluble aprotic organic compound, and 2% of CE510 (JSR Corporation) were mixed. Furthermore, 10% of sodium azide was added as a preservative to make it 0.05%, and mixed, and the reagent composition of Examples 9-14 was obtained.

[0098] Add 2% water-soluble polymer, 5mM metal salt, 1% surfactant, 0.95% isopropanol, 2% CE510 and 0.05% sodium azide and mixed to obtain the reagent composition of Comparative Examples 5-9.

[0099] [Table 2]

[0100]

[0101] [6. Measurement]

[0102] Using the chromatographic medium prepared above, the presence or absence of influenza A virus in the sample was determined by the following method. That is, when the gene of influenza A virus extracted from the nasal mucus of influenza virus-infected patient...

Embodiment 15 and comparative example 10～13

[0131] As recorded in Table 5, 1% of 10% Tween20, 5mM of 0.1M magnesium sulfate, 0.95% of dimethyl sulfoxide, 2% of 20% dextran sodium sulfate (weight average) were added to ultrapure water. Molecular weight: 500,000), and 2% of CE510 (JSR Corporation) and mixed. Furthermore, 10% of sodium azide was added and mixed as a preservative to 0.05%, and the reagent composition of Example 15 and Comparative Examples 10-13 was obtained.

[0132] [table 5]

[0133] Element

Amount added

Example 15

Comparative Example 10

Comparative Example 11

Comparative Example 12

Comparative Example 13

Sodium Dextran Sulfate

2％

○

○

○

○

magnesium sulfate

5mM

○

○

○

○

DMDO

0.95％

○

○

○

○

Tween 20

１％

○

○

○

○

[0134] [6. Measurement]

[0135] Using the chromatographic medium prepared above, the presence or absence of influenza A virus...

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PUM

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Abstract

Provided is a reagent composition capable of determining an analyte accurately and rapidly even when it has a low concentration in the measurement by nucleic acid chromatography or immunochromatography, by reducing the binding of components other than the analyte through a non-specific reaction and enhancing the dispersion capability of the analyte to improve the developability on a chromatography carrier and promote a specific reaction. A reagent composition for nucleic acid chromatography or immunochromatography includes a water-soluble polymer having a weight average molecular weight of 8,000 or more, a salt of a divalent or trivalent metal, a nonionic surfactant, and an aprotic water-soluble organic compound.

Description

technical field [0001] The present invention relates to a reagent composition used in nucleic acid chromatography or immunochromatography, a nucleic acid chromatography or immunochromatography measurement method using the same, and a nucleic acid chromatography or immunochromatography measurement kit containing the same. Background technique [0002] Conventionally, in the measurement of nucleic acid chromatography or immunochromatography, in order to increase the sensitivity, it is common practice to increase the amount of antibody or nucleic acid coated on a solid phase or to increase the amount of antibody or nucleic acid bound to a label. [0003] However, there are problems in that if reactants such as antibodies and nucleic acids are increased, non-specific reactions are likely to occur, and sufficient sensitivity cannot be obtained if the amount is small. [0004] In nucleic acid chromatography or immunochromatography, various reagent components are used for various p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCC07K16/1018C12Q1/6804G01N33/54393G01N33/558G01N30/00G01N2333/11Y10T436/143333C12Q2527/137G01N33/54387G01N33/58G01N33/54388
Inventor 榊原雄广
Owner TANAKA PRECIOUS METAL IND
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