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Heavy-metal-resisting microbial agent, preparation method and application thereof

A technology of microbial preparations and heavy metals, applied in the field of environmental biology, can solve problems such as rising, non-heavy metal indicators have not been improved, and water quality discharge standards cannot be met, and achieve the effect of low cost and high efficiency

Active Publication Date: 2013-05-08
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its essence is a physical and chemical effect, which is similar to the traditional physical and chemical method. Although the result can remove most of the heavy metals, the indicators of non-heavy metals such as organic matter have not been improved but have increased, which cannot meet the new water quality discharge standards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Collect 3 soil samples 3-10 cm below the surface layer polluted by heavy metals from different locations near the electroplating factory, carry out enrichment culture in shake flasks in the enrichment medium for 3 days, transfer culture for 3 times, and then dilute and plate in the separation medium , two single colonies were obtained after multiple isolations.

[0031] Two strains of bacteria were identified using the automatic bacterial identification system-Cell Fatty Acid Mapping Identification System (MIDI). After identification, the two strains were Acinetobacter calcoaceticus and Pseudomonas aeruginosa, and were preserved in the The General Microbiology Center of the China Committee for the Collection of Microbial Cultures is located at the Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing.

[0032] Acinetobacter calcium acetate is named Acinetobacter calcoacetcus (Acinetobacter calcoacetcus) HDD1, and the preservation...

Embodiment 2

[0035] Use beef extract peptone medium, the formula is beef extract 3g, peptone 10g, NaCl 5g, water 1000mL, pH7.4~7.6, medium contains Ni 2+ 50mg / L, Pb 2+ 20mg / L, Cr 6+ 10mg / L, Cu 2+ 10mg / L, Zn 2+ 500mg / L. Acinetobacter calcoacetcus (Acinetobacter calcoacetcus) with the preservation number CGMCC No.6820 and Pseudomonas aeruginosa (Pseudomonas aeruginosa) with the preservation number CGMCC No.6821 were respectively activated by slant culture at 28°C for 12 hours, and then in 500ml triangular The flask shaker was cultured separately, the culture time was 48 hours, and 3L of the bacterial liquid was obtained respectively; the 6L of the above-cultured bacterial liquid mixture was inoculated into a 20L solid fermenter, the inoculation amount was 30%, and the solid medium was composed of 50 parts of bran, corn 10 parts of core powder, 15 parts of soybean meal, 2 parts of starch, 2 parts of urea, 0.5 parts of sodium glutamate, 2 parts of ammonium sulfate, KH 2 PO 40.8 parts, th...

Embodiment 3

[0037] Use beef extract-peptone medium, the formula is beef extract 3g, peptone 10g, NaCl 10g, water 1000mL, pH7.4~7.6, medium contains Ni 2+ 50mg / L, Pb 2+ 20mg / L, Cr 6+ 10mg / L, Cu 2+ 10mg / L, Zn 2+ 100mg / L. The Acinetobacter calcoacetcus (Acinetobacter calcoacetcus) with the preservation number CGMCC No.6820 and the Pseudomonas aeruginosa (Pseudomonas aeruginosa) with the preservation number CGMCC No.6821 were respectively activated by slant culture at 28°C for 12 hours, and then in 500ml The Erlenmeyer flask shaker is cultured separately, and the culture time is 48h, and 3L of bacterial liquid is obtained respectively; 6L of the bacterial mixture cultured above is inoculated into a 20L solid fermenter, the inoculation amount is 30%, and the solid medium composition is in parts by weight: bran 50 parts, 10 parts of corn cob flour, 15 parts of soybean meal, 2 parts of starch, 2 parts of urea, 0.5 parts of sodium glutamate, 2 parts of ammonium sulfate, KH 2 PO 4 0.8 parts,...

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Abstract

The invention discloses a heavy-metal-resisting microbial agent, a preparation method and an application thereof. The preparation method of the microbial agent comprises the following steps of: (1) respectively carrying out liquid culture in a fluid medium after activating acinetobacter calcoacetcus with the preservation number of CGMCC No.6820 and pseudomonas aeruginosa with the preservation number of CGMCC No.6821 to obtain bacterial liquid A and bacterial liquid B; and (2) inoculating the mixed bacterial liquid A and B to a solid fermentation medium, ventilating, stirring and fermenting for 24-48 hours at 28-38 DEG C, and drying in vacuum to obtain the heavy-metal-resisting microbial agent. The heavy-metal-resisting microbial agent disclosed by the invention can not only remove a part of heavy metal ions, but also can remove a major of organic pollutants.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a heavy metal-resistant microbial bacterial agent and its preparation method and application. Background technique [0002] Cr 6+ 、Cu 2+ 、Ni 2+ , Zn 2+ Heavy metal ions, such as heavy metal ions, are biologically toxic and directly discharged into water bodies will have a great impact on the environment. They have always been the key targets of environmental protection monitoring at home and abroad. Microbial treatment of heavy metal wastewater has the characteristics of low cost, and has become a hot spot in the research of heavy metal wastewater treatment in recent years. [0003] The Chinese invention patent document with application number 03821202.1 discloses a kind of bacterium obtained after acid treatment with Bacillus KRI-02 or its similar bacterial strains, Bacillus licheniformis and Staphylococcus KRI-04 or its similar bacterial strains. Heavy metal sorb...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/385C02F103/16C02F103/24
Inventor 李明智张刚梅荣武韦彦斐王慧荣沈浙萍李欲如王付超蒋涛
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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