Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody

A VHPSMA-L-VLFITC, bispecific antibody technology, applied in the field of anti-PSMA/FITC bispecific antibody and its preparation, can solve the problems of poor stability of polyploid hybridoma cells, time-consuming and laborious, and achieve large-scale convenience. Production and preparation, strong penetration, small molecular weight effect

Inactive Publication Date: 2013-05-08
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method must screen a bispecific BsAb from a large number of similar immunoglobulins, which is time-consuming and laborious. At the same time, polyploid hybridoma cells have poor stability and require frequent cloning.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody
  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody
  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention will be described in detail below in combination with specific embodiments.

[0028] Bispecific antibody (bispecific antibody, BsAb) refers to an antibody with two antigen-binding properties. The two antigen-binding sites of this type of antibody can simultaneously bind to two different antigens or antigenic determinants, one of which can bind to the surface antigen of the target cell, and the other can bind to the effector (such as drug, effector cell etc.) combined. In fact, the bispecific antibody is a hybrid molecule, the structure of the two heavy chains is different from that of the two light chains, and the two Fab fragments are also different.

[0029] PSMA is a type II intrinsic membrane protein present in the prostate gland epithelial cell membrane, which has high tissue specificity. As early as 1998, Bostwick et al. found in the immunological study of 187 prostate specimens that from benign prostate epithelium In the highly malignant c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a bispecific antibody of resisting PSMA / FITC (prostate specific membrane antigen / fluorescein isothiocyanate) for early diagnosis and treatment of a prostatic cancer. A single-chain antibody about the PSMA has been reported more at present, but the bispecific antibody of resisting the PSMA / FITC is researched less. The bispecific antibody related to the invention has a full-length nucleotide sequence shown in SEQ ID No.5. The bispecific antibody is produced by a gene recombination technology, and is convenient to produce and prepare at a large scale, small in molecular weight of an antibody, and strong in penetrating power of tumor tissue; micromolecule antibody can easily achieve the diseased region aiming at the anatomical features that the prostate tissue has thick prostate envelope; and treatment and early diagnosis of the prostatic cancer can be achieved by drugs carried by hapten or a marker by combining with prostatic cancer cells of the diseased region by a pre-targeting technology.

Description

technical field [0001] The invention relates to an antibody, in particular to an anti-PSMA / FITC bispecific antibody for early diagnosis and treatment of prostate cancer and a preparation method thereof. Background technique [0002] Prostate cancer is one of the most common malignant tumors in European and American countries, and it ranks second in the incidence of male tumors in Europe and America. In the United States, the incidence of prostate cancer accounts for the first of all malignant tumors, and the mortality rate is second only to lung cancer. In recent years, the incidence of prostate cancer in China has also shown a gradual upward trend. The early diagnosis of prostate cancer has been paid more and more attention by scholars. Following the discovery of prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA), which were widely used in the diagnosis and treatment of prostate cancer, a prostatic cancer that exists in the LNCaP line The glycoprotein i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/46C12N15/13C12N15/70G01N33/68G01N33/574
Inventor 宦怡魏梦绮袁远郑敏文张劲松杨勇任静黄旭方赵亚超李昕宇
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com