Method for converting ferulic acid to produce vanillin by immobilized amycolatopsis

A technology of Amycolatopsis and ferulic acid, applied in the field of bioengineering, can solve the problems of low conversion rate of ferulic acid, low production efficiency, high production cost, etc., and achieve continuous production, low cost and high production cost. simple effect

Inactive Publication Date: 2013-04-24
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these conversion processes have a long cycle and low production efficiency; the conversion rate of raw material ferulic acid is not high (molar conversion rate 20%-70%), and the production cost is relatively high

Method used

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  • Method for converting ferulic acid to produce vanillin by immobilized amycolatopsis
  • Method for converting ferulic acid to produce vanillin by immobilized amycolatopsis
  • Method for converting ferulic acid to produce vanillin by immobilized amycolatopsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0021] Example 1-4, the situation of vanillin produced by immobilized cells prepared from sawdust with different treatment methods

[0022] Sawdust is processed as follows: soak and rinse with clean water, H 2 O 2 Treatment; NaOH water bath; NaOH boiling. Take a ring of bacterial lawn from the preservation slant and inoculate it into the seed medium, the composition of the seed medium: glucose 5g; yeast extract 5g; Na 2 HPO 4 ·12H 2 O4g; KH 2 PO 4 1g; NaCl0.2g; MgSO 4 ·7H 2 O0.2g; CaCl 2 2H 2 O0.05g; dilute to 1L with tap water, adjust the pH to 7.2, and fill in a 30mL / 250mL triangular flask. Cultivate in a constant temperature shaker at 30°C and 200r / min for 24 hours; inoculate the seeds into the cell expansion medium with an inoculum of 8% (the composition of the expansion medium is the same as that of the seed medium, and the liquid volume is 50mL / 500mL Erlenmeyer flask), Expand culture at 37°C and 200r / min for 24h, add 10g of treated sawdust, continue to cultiva...

Embodiment 5-9

[0025] Embodiment 5-9 temperature produces vanillin situation to immobilized cell transformation

[0026] Immobilized cells were prepared according to the method in Example 1, and transformed at different transformation temperatures: 28°C, 30°C, 35°C, 37°C, and 40°C. The results are shown in Table 2:

[0027] Table 2 The influence of temperature on the production of vanillin by transformation of immobilized cells

[0028]

[0029] The conversion reaction is carried out at 35-40°C.

Embodiment 10

[0030] Example 10 Reuse of immobilized cells

[0031] Prepare immobilized cells according to the method of Example 1, the substrate ferulic acid concentration of the transformation reaction is 10g / L, the initial transformation pH is 9.0, the transformation temperature is 40°C, the shake flask rotation speed is 200rpm, the transformed immobilized cells are filtered, and repeated Use, see Table 3 for each result:

[0032] Table 3 Reuse of immobilized cells

[0033]

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Abstract

The invention discloses a method for converting ferulic acid to produce vanillin by immobilized amycolatopsis, and belongs to the technical field of biology engineering. Cellulosic materials such as sawdust, bagasse and the like are used as carriers, and an amycolatopsis cell is fixed by an adsorption way. An immobilized cell can be recycled for 10 times, and the mole conversion rate for converting ferulic acid into vanillin is kept at more than 50%. A resin is coupled and adsorbed in a conversion system. The output of vanillin reaches 19.65g/L, and the production strength of vanillin is 0.131g/L.h. The method is mild in conversion reaction condition, little in environmental pollution, short in production period, low in cost and easily separates and purifies converted products, so that the method has a good application prospect.

Description

technical field [0001] The invention relates to the immobilization of a strain of Amycolatopsis sp. zhp06, namely CCTCC NO: M2011265, and a biotransformation method for preparing vanillin by transforming ferulic acid, belonging to the technical field of bioengineering. Background technique [0002] Vanillin (3-methoxy-4-hydroxybenzaldehyde), also known as vanillin and citronellal, has a relative molecular weight of 152.15, white to slightly yellow needle-like or powdery crystals, and is the main component of vanilla. Used in food, beverage, fragrance, medicine, leather, electroplating and pharmaceutical industries. [0003] The production method of vanillin is mainly a chemical synthesis method, and its main source is the chemical synthesis of petrochemical products guaiacol, lignin, etc. The vanillin synthesized by this method not only has a single fragrance type, but also has relatively high environmental pollution during the synthesis process. Seriously, people are incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/24C12N11/12C12R1/01
Inventor 郑璞王兴林
Owner JIANGNAN UNIV
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