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Making method of ceramide and/or glucosylceramide generation accelerator

A technology of ceramide and manufacturing method, applied in the field of ceramide and/or glucosylceramide production accelerator, to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2013-04-24
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Also, there is no report on the use of microorganisms of the genus Brettanomyces to produce ceramide and / or glucosylceramide production accelerators

Method used

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  • Making method of ceramide and/or glucosylceramide generation accelerator

Examples

Experimental program
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Effect test

Embodiment 1

[0048] (Manufacture of ceramide and / or glucosylceramide production promoter)

[0049] As a microorganism for producing ceramide and / or glucosylceramide production accelerator from bean dregs, Brettanomyces claussenii (purchased from Jiangnan University and deposited in CICIM, China Universities Industrial Microbiology Resources and Information Center, deposit No. CICIM Y0125).

[0050] Inoculate the above strain obtained by culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C into a Erlenmeyer flask (capacity 300ml) containing 100ml of ceramide / glucosylceramide production promoter production medium, and inoculate at 30°C cultured for 72 hours. The medium contained 10 w / v% okara and 1 w / v% glucose. Wherein, the preparation of soybean dregs is obtained by soaking soybeans in water for 12 hours to make soybeans fully absorb water, then grinding and crushing the soybeans that have absorbed water, filtering and removing liquid components, and draining remaining...

Embodiment 2

[0054] As a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs, Brettanomyces claussenii (purchased from Jiangnan University and deposited in CICIM, China Universities Industrial Microbiology Resources and Information Center, accession number CICIMY0251 ). The same method as in Example 1 was used to obtain the okara fermented extract as the ceramide / glucosylceramide production accelerator of the present invention.

[0055] Furthermore, this okara fermented extract was dissolved in 50v / v% ethanol, adjusted to a concentration of 0.1w / v% (product B of the present invention), stored at 4°C, and used for the subsequent promotion of ceramide / glucosylceramide production test.

Embodiment 3

[0057] As a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs, Brettanomyces claussenii (purchased from Jiangnan University and deposited in CICIM, China Universities Industrial Microbiology Resources and Information Center, accession number CICIM Y0127). The same method as in Example 1 was used to obtain the okara fermented extract as the ceramide / glucosylceramide production accelerator of the present invention.

[0058] Furthermore, the okara fermented extract was dissolved in 50v / v% ethanol, and the concentrations were adjusted to 1w / v% (invention product C) and 0.1w / v% (invention product D), respectively, and stored at 4°C , used in the subsequent ceramide / glucosylceramide production promotion test.

[0059] (Ceramide / glucosylceramide production promotion test)

[0060]

[0061] First, normal human activated keratinocytes (Cascade Co., Ltd) were used as primary cells, and placed in a 75cm 2 In a culture flask (2ml of culture ...

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Abstract

The invention provides a making method of a ceramide and / or glucosylceramide generation accelerator. Specifically, a Brettanomyces microorganism is cultivated in a medium containing bean dregs and saccharides, and the ceramide and / or glucosylceramide generation accelerator can be acquired from the supernatant of the medium. According to the making method provided in the invention, the by-product bean dregs from the food processing industry and the like can be taken as the main raw material of the medium, so that the ceramide and / or glucosylceramide generation accelerator can be efficiently obtained at a low cost.

Description

technical field [0001] The present invention relates to a method for producing a ceramide and / or glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin by using specific microorganisms and using bean dregs, a by-product of the food processing industry, etc., as the main raw material of a culture medium . Background technique [0002] Ceramide, one of the sphingolipids, exists in the stratum corneum of the skin and accounts for about 50% of the intercellular lipids in the stratum corneum. Sphingolipid is a general term for complex lipids having a sphingosine skeleton. Glucosylceramide is a kind of sphingolipid formed by binding glucose to ceramide. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (Glucocerebrosidase) and sphingomyelinase (Sphingomyelinase) Under the action of cera...

Claims

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Application Information

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IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/645
Inventor 孔凡旗
Owner KAO CORP
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