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Manufacturing method of ceramide production promoter

A technology of ceramides, manufacturing methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, preparations for skin care, etc., to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2013-03-27
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, there is no report of using fission yeast to prepare a ceramide production accelerator or a glucosylceramide production accelerator

Method used

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  • Manufacturing method of ceramide production promoter

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Effect test

Embodiment 1

[0044]

[0045] First, the culture medium is prepared according to the following method: Soak soybeans in water for 12 hours to allow the soybeans to fully absorb water, then grind and crush the absorbed soybeans, filter them to remove liquid components, and drain the remaining residues to obtain bean dregs. The okara was mixed with glucose and water, and the concentrations of the okara and glucose in the mixture were respectively 10w / v% and 1w / v%, and the mixture was used as a culture medium.

[0046] Then, add 100 ml of the above-mentioned culture medium to the Erlenmeyer flask, inoculate a certain amount of Schizosaccharomyces octoporus (preserved in CICIM, Industrial Microbiology Resource and Information Center, Jiangnan University, No. CICIM Y0123), and cultivate at 30°C 3 days.

[0047] The resulting culture was then centrifuged (3000 rpm, 10 minutes). The remaining supernatant was diluted with 2.5 times of absolute ethanol, and the mixture diluted with absolute ethan...

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Abstract

The invention provides a manufacturing method of a ceramide production promoter and / or a glucose ceramide production promoter, wherein the ceramide production promoter and / or the glucose ceramide production promoter is obtained from supernatant fluid of a microorganism of Schizosaccharomyces, which is cultured in a culture medium containing bean dregs and saccharides. According to the manufacturing method disclosed by the invention, substances which are easy to obtain can be used as the culture medium, the specific microorganism can be further utilized, and then the ceramide production promoter and / or the glucose ceramide production promoter can be obtained at a low cost with high efficiency.

Description

technical field [0001] The present invention relates to a method for producing a ceramide production promoter and / or a glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin. Background technique [0002] The skin has the ability to produce ceramide and glucosylceramide. In the skin, especially in the stratum corneum of the epidermis, ceramide synthesized and secreted by epidermal cells is accumulated, which is the main component of intercellular lipids. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (glucocerebrosidase) and sphingomyelinase (sphingomyelinase) ) to form ceramide again, thereby functioning as an intercellular lipid. Ceramides and glucosylceramides in the skin have physiological effects of improving the moisturizing function of the skin, the barrier function of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/645
Inventor 孔凡旗
Owner KAO CORP
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