Novel method for effectively extracting lentinan
A high-efficiency technology of lentinan, applied in the field of plant active ingredient extraction, can solve the problems of low extraction rate, large loss of active ingredients, long cycle and the like, and achieves the effects of simple operation, improved extraction efficiency and low cost
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Embodiment 1
[0034] Get the dried shiitake mushroom fruiting bodies, wash and remove visible impurities therein, pulverize with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 300 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 5 minutes to release the extract from the lower end of the extraction tank, then add 300 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut-off of 50,000 until the weight of the filtrate is 2.0 times that of the mushroom powder. Add 95% ethanol pre-cooled to 4°C in the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, recover ethanol from the supernatant for ...
Embodiment 2
[0037] Take the dried shiitake stalks, wash and remove visible impurities therein, pulverize them with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 200 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 6 minutes to release the extract from the lower end of the extraction tank, then add 200 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut off of 50,000 until the weight of the filtrate is 1.5 times the weight of the mushroom powder. Add 95% ethanol pre-cooled to 4°C in the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, recover ethanol from the supernatant for recycl...
Embodiment 3
[0040] Get the dried shiitake mushroom fruiting bodies, wash and remove visible impurities therein, pulverize with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 350 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 6 minutes to release the extract from the lower end of the extraction tank, then add 350 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut off of 50,000 until the weight of the filtrate is 1.5 times the weight of the mushroom powder. Add 80% edible ethanol pre-cooled to 4°C into the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, and recover the ethanol fr...
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