Polypeptide having high affinity with receptor of integrin alpha v beta3
An integrin and affinity technology, applied in the direction of peptides, drug combinations, tripeptide components, etc., to achieve the effect of low synthesis cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] Synthesis of RWR
[0023] First use R-D-Arg (fluorenylmethoxycarbonyl-arginine-king resin) 0.36 / 2mmol5.55g, add raw material L-Trp2.106g, use HBTU[2-(7-azobenzotriazole )-Tetramethyluronium hexafluorophosphate] 1.44g reaction time half an hour, cap removal time 40 minutes, then add raw material L-Arg 2.6g, condensing agent HBTU1.44g reaction time half an hour, cap removal time 40 minutes. Then purify : Dissolve the sample in acetonitrile, load the sample to the C18 preparative column, perform gradient elution with HPLC, collect the liquid, and test the purity of the liquid. Qualified samples were rotary evaporated and freeze-dried in a lyophilizer to obtain 200 mg of RWR.
[0024] The sequence is: Arg-Trp-Arg
[0025] It can be seen from the mass spectrum that the molecular weight of RWR is 517.61-1=516.61. There is an absorption peak of the peptide at 284nm in the ultraviolet spectrum.
Embodiment 2
[0027] Synthesis of RKY
[0028] First use R-D-Tyr (fluorenyl methaneoxycarbonyl-arginine-king resin) 0.33 / 2mmol6.06g, add raw material D-Lys1.88g, condensing agent with HBTU1.44g reaction time 25 minutes, uncapping time 30 minutes, and then Add 2.6g of D-Arg as raw material, 1.44g of HBTU as condensing agent, the reaction time is half an hour, and the capping time is 30 minutes. Then purify: dissolve the sample in acetonitrile, load the sample to the C18 preparative column, use HPLC for gradient elution, collect the liquid, and detect liquid purity. Qualified samples were rotary evaporated and freeze-dried in a lyophilizer to obtain 200 mg of RKY.
[0029] The sequence is: Arg-Lys-Tyr.
[0030] It can be seen from the mass spectrum that the molecular weight of Arg-Lys-Tyr is 466.56-1=465.56. There is an absorption peak of the peptide at 273nm in the ultraviolet spectrum.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com