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Method for preparing astaxanthin extractive from haematococcus pluvialis powder

A technology of Haematococcus pluvialis powder and astaxanthin, applied in the direction of organic chemistry, etc., can solve the problems of hindering large-scale industrial production, large amount of carbon dioxide consumption, low astaxanthin content, etc., and achieve good promotion and application prospects and reduce energy consumption. The effect of reduction and simple process

Inactive Publication Date: 2013-04-17
NINGBO HONGLONG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of these solvents are toxic reagents with high prices, and there are potential safety hazards in the production process and products
Although supercritical carbon dioxide extraction can effectively extract astaxanthin and avoid its degradation to the greatest extent, it requires high equipment, is expensive, and consumes a large amount of carbon dioxide. The content of astaxanthin in the extract is low, which hinders its large-scale production. scale industrial production

Method used

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  • Method for preparing astaxanthin extractive from haematococcus pluvialis powder

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, a kind of method for preparing astaxanthin extract from Haematococcus pluvialis powder, carries out following steps successively:

[0031] 1) Weigh 0.1g of Haematococcus pluvialis powder, add 1mL of 95% food-grade alcohol, mix well and use it as a reaction system in a dark place for extraction at room temperature (25-30°C) for 1 hour, and react every 15 minutes Mix the system once;

[0032] 2) After the extraction, centrifuge at 2000rpm for 15min to obtain the residue and supernatant respectively;

[0033] 3) Replace the Haematococcus pluvialis powder in step 1) with the residue, and repeat step 1) and step 2) twice in total;

[0034] That is, in the present embodiment, the Haematococcus pluvialis powder has been extracted 3 times;

[0035] 4) Combine the supernatant obtained in step 2) and the second supernatant obtained in step 3); concentrate under reduced pressure at 35°C to recover alcohol, and obtain 20 mg of dark red extract (astaxanthin extract)....

Embodiment 2

[0039] Embodiment 2, a kind of method for preparing astaxanthin extract from Haematococcus pluvialis powder, carries out following steps successively:

[0040] 1) Weigh 1g of Haematococcus pluvialis powder, add 20mL of 95% food-grade alcohol, mix well, and use it as a reaction system, place it in a dark place for extraction at room temperature (30°C) for 2 hours, and mix it every 20 minutes ;

[0041] 2) After the extraction, centrifuge at 3000rpm for 10min to obtain the residue and supernatant respectively;

[0042] 3) Replace the Haematococcus pluvialis powder in step 1) with the residue, and repeat step 1) and step 2) twice in total;

[0043] That is, in the present embodiment, the Haematococcus pluvialis powder has been extracted 3 times;

[0044] 4) Combine the supernatant obtained in step 2) and the second supernatant obtained in step 3); concentrate and recover alcohol under reduced pressure at 45°C to obtain a dark red extract, which is astaxanthin extract.

[0045] T...

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Abstract

The invention discloses a method for preparing an astaxanthin extractive from haematococcus pluvialis powder. The method comprises the following steps: (1), weighing haematococcus pluvialis powder, adding food-grade alcohol, fully and uniformly mixing to obtain a mixture as a reaction system, and extracting the reaction system for 1 to 2 hours at a room temperature in a dark place, wherein the ratio of haematococcus pluvialis powder to food-grade alcohol is 1 g / 10-20 mL, and the food-grade alcohol adopts a food-grade alcohol solution with the volume concentration of larger than or equal to 95%; (2), centrifuging after extraction is finished to respectively obtain residue and supernatant liquor; (3) replacing the haematococcus pluvialis powder obtained from the step (1) with the residue, and repeating the step (1) and the step (2) at least once in sequence; and (4), combining the supernatant liquor obtained from the step (2) and the supernatant liquor obtained from the step (3), and recovering alcohol through vacuum concentration to obtain a dark red extract, namely the astaxanthin extractive. The astaxanthin extractive, prepared by the method, has the characteristic of high content of the astaxanthin monomer.The invention discloses a method for preparing an astaxanthin extractive from haematococcus pluvialis powder. The method comprises the following steps: (1), weighing haematococcus pluvialis powder, adding food-grade alcohol, fully and uniformly mixing to obtain a mixture as a reaction system, and extracting the reaction system for 1 to 2 hours at a room temperature in a dark place, wherein the ratio of haematococcus pluvialis powder to food-grade alcohol is 1 g / 10-20 mL, and the food-grade alcohol adopts a food-grade alcohol solution with the volume concentration of larger than or equal to 95%; (2), centrifuging after extraction is finished to respectively obtain residue and supernatant liquor; (3) replacing the haematococcus pluvialis powder obtained from the step (1) with the residue, and repeating the step (1) and the step (2) at least once in sequence; and (4), combining the supernatant liquor obtained from the step (2) and the supernatant liquor obtained from the step (3), and recovering alcohol through vacuum concentration to obtain a dark red extract, namely the astaxanthin extractive. The astaxanthin extractive, prepared by the method, has the characteristic of high content of the astaxanthin monomer.

Description

technical field [0001] The invention belongs to the extraction and development of natural products, and mainly relates to a method for preparing astaxanthin extract from Haematococcus pluvialis powder by alcohol extraction. Background technique [0002] Astaxanthin is currently the carotenoid with the strongest antioxidant activity, and its antioxidant activity is more than 500 times that of vitamin E and β-carotene. Astaxanthin also has the functions of anti-tumor, lowering blood fat, delaying aging, etc. It is widely used in food, cosmetics and other industries, and has a broad market prospect. At the same time, as a fat-soluble bright red pigment, it can color a variety of foods in the food industry and increase the color and beauty of food; when used in ornamental fish and shrimp farming, it can also increase color, enhance the appearance, and increase its economic value. Astaxanthin is water-insoluble and lipophilic, soluble in organic solvents such as pyridine, benzen...

Claims

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Application Information

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IPC IPC(8): C07C403/24
Inventor 黄胜奎马建陈黎明
Owner NINGBO HONGLONG BIO TECH
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