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Horse bone protein powder rich in active calcium and preparation method thereof

A technology of active calcium and protein powder, used in food preparation, protein food processing, protein food ingredients and other directions, can solve the problems of calcium deficiency, unable to find calcium source, pollute the environment, etc., achieve good processing characteristics, promote human absorption, The effect of high water solubility

Inactive Publication Date: 2013-04-17
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing practice only focuses on the consumption and production of horse meat and horse urine, and a large number of horse bones are not fully utilized, which not only wastes this resource rich in nutrients and has a reasonable proportion, but also has problems in bone treatment. Environmental pollution has brought certain environmental pressure; at the same time, some people have long-term serious calcium deficiency, but they have not been able to find an economical and effective calcium source to supplement

Method used

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  • Horse bone protein powder rich in active calcium and preparation method thereof
  • Horse bone protein powder rich in active calcium and preparation method thereof
  • Horse bone protein powder rich in active calcium and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: the preparation of rich active calcium horse bone protein powder

[0031] The preparation method of active calcium-rich horse bone protein powder, the specific preparation method steps are as follows:

[0032] (1) The fleshless raw horse bone is crushed to 10cm, soaked to remove blood, and dried at 50°C to obtain massive horse bone;

[0033] (2) Preparation of degreasing horse bone: using n-hexane as the degreasing reagent and total protein content as the index, select the solid-liquid ratio of massive horse bone and n-hexane as 1:5g / mL, degreasing temperature at 40°C, and degreasing time for 8h to obtain degreasing horse bone;

[0034] (3) Preparation of horse bone powder hydrolyzate: pulverize the defatted horse bone prepared in the above step (2), and sieve through 120 mesh to obtain bone powder; select alkaline protease, enzymolysis temperature is 45°C, and the concentration of bone powder aqueous solution is in m / m 8%, the enzymolysis pH value is ...

Embodiment 2

[0037] Embodiment two: the preparation of rich active calcium horse bone protein powder

[0038] The preparation method of active calcium-rich horse bone protein powder, the specific preparation method steps are as follows:

[0039] (1) The fleshless raw horse bone is broken to 10cm, soaked to remove blood, and dried at 55°C to obtain massive horse bone;

[0040] (2) Preparation of degreasing horse bone: using n-hexane as the degreasing reagent and total protein content as the index, select the solid-liquid ratio of massive horse bone and n-hexane as 1:4g / mL, degreasing temperature at 45°C, and degreasing time for 10h to obtain degreasing horse bone;

[0041] (3) Preparation of horse bone powder hydrolyzate: the degreased horse bone prepared in the above step (2) is pulverized and sieved with 120 mesh to obtain bone powder; alkaline protease is selected, the enzymolysis temperature is 55°C, and the concentration of the bone powder aqueous solution is m / m 10%, the enzymolysis...

Embodiment 3

[0043] Embodiment three: the preparation of active calcium horse bone protein powder

[0044] The preparation method of active calcium-rich horse bone protein powder, the specific preparation method steps are as follows:

[0045] (1) The fleshless raw horse bone is crushed to 10cm, soaked to remove blood, and dried at 50°C to obtain massive horse bone;

[0046] (2) Preparation of degreasing horse bone: using n-hexane as the degreasing reagent and total protein content as the index, select the solid-liquid ratio of massive horse bone and n-hexane as 1:3g / mL, degreasing temperature at 43°C, and degreasing time for 9h to obtain degreasing horse bone;

[0047] (3) Preparation of horse bone powder hydrolyzate: the degreased horse bone prepared in the above step (2) is pulverized and sieved with 120 mesh to obtain bone powder; alkaline protease is selected, the enzymolysis temperature is 50°C, and the concentration of the bone powder aqueous solution is m / m 9%, the enzymolysis pH ...

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Abstract

The invention discloses horse bone protein powder rich in active calcium and a preparation method thereof. The horse bone protein powder rich in active calcium is obtained by crushing and drying raw horse bones, degreasing for 8-10 h through taking n-hexane as a degreasing reagent at the temperature of 40 to 45 DEG C and a feed liquid ratio of 1:3g / mL-1:5g / mL, and grinding and screening the degreased bone powder; selecting an alkaline protease for enzymolysis at a temperature of 45 to 55 DEG C, controlling the concentration of the bone powder to 8% to 10%, the pH value to 9, the enzyme concentration to 5,000U / g to 6,000U / g, the time to 3-5h and temperature to 90 DEG C, carrying out enzyme deactivation and cooling for 10 min, centrifuging at a speed of 4,000r / min to 6,000r / min, and filtrating; precipitating bone residues, drying at a temperature of 50 DEG C, calcining for 2-3h at a temperature of 850 to 900 DEG C, activating with ethanol at a temperature of 110 to 120 DEG C, and mixing, homogenizing and drying horse bone enzymolytic supernatant and activated calcium in a feed liquid ratio of 1:4g / mL-1:6g / mL. The prepared horse bone protein powder rich in active calcium has extensive application value.

Description

field of invention [0001] The invention relates to the field of horse bone processing, in particular to a preparation process of horse bone protein bone powder with high activity calcium. Background technique [0002] There has been a history of 70 years of processing and utilization of livestock and poultry bone waste abroad, and developed countries, especially Sweden, Denmark, Japan, and the United States, have been active in the development and utilization of bone (Bhaskar et al., 2007). Ye Mingquan et al. (1999) reported that as early as the 1970s, countries such as Sweden and Denmark had developed bone processing equipment to produce bone paste and other products; in the 1980s, the United States developed bone into soluble protein for research and utilization (Zhao Xia and Ma Lizhen, 2003); In the 1990s, Japan developed bones into a series of products such as bone pine, bone flavored juice, and bone flavored ingredients, and added them to soups and condiments for use (L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/312A23J3/34A23L33/00A23L13/20
Inventor 杨海燕秦娜娜侯伟伟
Owner XINJIANG AGRI UNIV
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