Long-distance transportation method of sepia esculenta adults
A technology for long-distance transportation of golden squid, applied in fish farming, application, climate change adaptation, etc., can solve problems such as no golden squid, achieve long survival time, avoid physical damage, and ensure the effect of transportation water quality
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Embodiment 1
[0026] (1) Stage according to the anesthesia and resuscitation behavior of golden squid, and then determine the appropriate anesthesia operation concentration and anesthesia transport concentration of golden squid
[0027] Experimental design 9 concentration gradients of 2, 5, 8, 15, 20, 30, 40, 60 and 80 mg / L, 5 adults of golden squid were tested at each concentration, the final degree of anesthesia reached by golden squid within 24 hours was determined, and the final anesthesia was observed. The behavioral characteristics in the state, and then transferred to clean seawater for resuscitation, the behavioral characteristics during the resuscitation process were observed, and then the degree of anesthesia and the resuscitation process were staged.
[0028] Test results: 1) According to the behavioral characteristics under the final anesthesia state, the degree of anesthesia was divided into six periods, as shown in Table 1.
[0029] Table 1 Stages of anesthesia degree and beha...
Embodiment 2
[0036] In June 2012, we transported 250 adult golden squid from Qingdao Jinshatan Aquatic Development Co., Ltd. to Tianjin Xinyongfeng Aquaculture Co., Ltd. Use ice cubes for transport water to cool down from 24°C to 18°C, add 7 mg / L MS-222 and then fill with nano-air stone to saturation; add ice cubes or ice bottles to the golden squid culture pond that has been fasted for 1 day to cool down, after 2 Step by step cooling treatment, the temperature difference should not exceed 3 °C each time, and the temperature was lowered to 18 °C; then MS-222 with a final concentration of 30 mg / L was added. The fins stopped swinging and the sense of balance was lost. Immediately, gently fish the golden squid into the washbasin. After weighing, slowly take it out by hand and put it into a black plastic bag with 10L of water for transportation. Each bag contains 1 adult golden squid. , squeeze out the air in the bag, fill with about 30L of oxygen, tie the bag with a rubber band and put it int...
Embodiment 3
[0038]In July 2012, we transported 150 adult golden squid from the Huangdao Golden Squid Experimental Base of the Yellow Sea Fisheries Research Institute to the Xinhaixin Nursery in Xiapu, Fujian. Use ice cubes for transport water to cool down to 16°C, add 8mg / L MS-222, and then use nano-air stone to fill with pure oxygen to saturation; add ice cubes or ice bottles to the golden squid culture pond that has stopped eating for 1 day to cool down, after 3 times The temperature was lowered to 16°C with a temperature difference of no more than 5°C each time; then MS-222 with a final concentration of 60 mg / L was added. After 3 minutes, the golden squid entered an anesthesia state for stage 4, that is, the wrists spread out and the lateral fins stopped. Swing, the sense of balance is lost. Immediately take the golden squid gently into the washbasin. After weighing, slowly take it out by hand and put it into a black plastic bag with 10L water for transportation. Put 1 adult golden squi...
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