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Boletus mother culture medium and preparation method thereof

A technology of mother seed culture medium and boletus, applied in fertilizer mixture, fertilization device, application, etc., can solve the problem of low fruiting body yield of culture materials, affecting the normal growth of boletus, and difficulty in growing the mother seed mycelium on the inclined surface of the test tube and other issues to achieve the effect of rapid growth

Inactive Publication Date: 2013-04-03
INST OF MOUNTAIN HAZARDS & ENVIRONMENT CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the boletus mycelium cultured in the traditional mother seed medium can obtain fruiting bodies in the cultivation material, it is difficult for the mother seed mycelium to cover the slope of the test tube, and the primordia will form on the 4th to 7th day of growth , and affect the normal growth of boletus in the future, and the fruiting body output of culture material output is low

Method used

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  • Boletus mother culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Weigh 18-24 parts of glucose, 5-8 parts of yeast powder, 2-4 parts of soybean powder, 0.2-0.6 parts

[0061] K H 2 PO 4 , 0.25-0.65 parts MgSO 4 ·H 2 O, put it in a container, add 200-300 parts of water, and place it for later use;

[0062] Put 15-23 parts of humus into a beaker, add 200-250 parts of water, boil and extract for 15-22 minutes, filter, and add the filtrate to the container; put 40-55 parts of dry pine needles into a beaker and add 200 -250 parts of water were boiled and extracted for 15-20 minutes, and the extract was added to the container;

[0063]Heat the above materials in the container for 1-3 minutes at a temperature of 90-100°C; then add 14-22 parts of agar, heat for 2-3 minutes until the agar is completely dissolved, add water to make up the volume, and divide into test tubes;

[0064] Sterilize at a temperature of 120° C., a pressure of 101.33 KPa, and a time of 30 minutes to obtain a boletus female culture medium.

Embodiment 2

[0066] Weigh 19-22 parts of glucose, 6-7.5 parts of yeast powder, 2.5-3.5 parts of soybean powder, 0.3-0.53 parts

[0067] K H 2 PO 4 , 0.4-0.6 parts MgSO 4 ·H 2 O, put it in a container, add 200-300 parts of water, and put it aside; put 18-21 parts of humus into a beaker, add 200-250 parts of water, boil and extract for 15-22 minutes, filter, add the filtrate into the container; put 48-52 parts of dry pine needles into a beaker, add 200-250 parts of water, boil and extract for 15-20 minutes, take the extract and add it to the container;

[0068] Heat the above materials in the container for 1-3 minutes at a temperature of 90-100°C; then add 16-21 parts of agar, heat for 2-3 minutes until the agar is completely dissolved, add water to make up the volume, and pack into test tubes;

[0069] Sterilize at a temperature of 120° C., a pressure of 101.33 KPa, and a time of 25 minutes to obtain a boletus female culture medium.

Embodiment 3

[0071] Will weigh 20 parts of glucose, 6.5 parts of yeast powder, 3 parts of soybean powder, 0.5 parts of KH 2 PO 4 , 0.45 parts MgSO 4 ·H 2 O, put it in a container, add 300 parts of water, and place it for later use; put 20 parts of humus into a beaker, add 200 parts of water, boil and extract for 15-22 minutes, filter, and add the filtrate to the container; Put one part of dry pine needles into a beaker and add 200 parts of water to boil and extract for 15-20 minutes, then take the extract and add it to the container;

[0072] Heat the above materials in the container for 1-3 minutes at a temperature of 90-100°C; then add 20 parts of agar, heat for 2-3 minutes until the agar is completely dissolved, add water to make up the volume, and divide into test tubes;

[0073] Sterilize at a temperature of 120° C., a pressure of 101.33 KPa, and a time of 30 minutes to obtain a boletus female culture medium.

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Abstract

The invention relates to the field of fungus culture media, in particular to a boletus mother culture medium and a preparation method thereof. The boletus mother culture medium comprises the following components in part by weight: 18-24 parts of glucose, 5-8 parts of yeast powder, 2-4 parts of soybean meal, 40.2-0.6 parts of KH2PO, 0.25-0.65 parts of MgSO4.H2O, 15-23 parts of humus, 40-55 parts of dry pine needles, 14-26 parts of agar and 800-1500 parts of water. The boletus mycelium is high in growth speed; during growth of the mycelium, a primordium is not formed; an excellent mother with high yield is provided for artificial cultivation of a sporocarp; on the 14-16th day, the mycelium can grow fully on a test tube slant with the length of 5-8cm; and compared with the conventional mother culture medium, the boletus mother culture medium can increase the yield of the boletus by 20-30%.

Description

technical field [0001] The invention belongs to the field of fungal culture medium, and in particular relates to a culture medium for boletus mother species and a preparation method thereof. [0002] Background technique [0003] Boletus is the general designation of fungi such as Boletaceae and Pine Cone Boletus, and most of them are edible except that a few varieties are poisonous or bitter and cannot be eaten. Some boletus are delicious and nutritious, such as delicious boletus, yellow-brown boletus and gray-brown boletus. Edible boletus generally has a large body, thick meat, strong stalk, sweet and delicious taste, and rich nutrition. It is a class of world-renowned edible fungi. [0004] Boletus is rich in mineral matter such as protein, carbohydrate, vitamin and calcium, phosphorus, iron. [0005] Boletus is a precious fungus. Some varieties of boletus have unique fragrance and rich nutrition. Some varieties of boletus have the functions of preventing and curing ...

Claims

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Application Information

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IPC IPC(8): C05G1/00
Inventor 张丹李付杰李伟
Owner INST OF MOUNTAIN HAZARDS & ENVIRONMENT CHINESE ACADEMY OF SCI
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