Novel strain of hypsizigus marmoreus
A technology of true mushroom and new strains, applied in the field of new strains of true mushroom, can solve the problems of long cultivation and cultivation cycle, weakening competitiveness, bitter taste, etc., shorten the cultivation and cultivation cycle, reduce production cost, keep fresh long term effect
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Embodiment 1
[0022] Embodiment 1 hybridization obtains Finc-B-1
[0023] This example illustrates the breeding process of the new strain Finc-B-1 of Shijiji mushroom. Specific steps are as follows:
[0024] 1) Select parental strains: select TNN-11 and Boduo A-264 as hybrid parents. The fruiting bodies of these two parents can be purchased in the market, and are also preserved in the strain collection center of Shanghai Academy of Agricultural Sciences.
[0025] 2) Preparation of culture medium
[0026] PDA medium: use 200g of potatoes, 20g of glucose and 20g of agar per 1L of medium.
[0027] Original seed medium: 55%-70% of wood chips, 15%-20% of rice bran, 5%-10% of bran, and 8-35% of corn flour are used as raw materials for cultivation. Add water to make the water content in the medium reach 60-62%.
[0028] Culture medium: 40-50% corn cob, 20-30% soybean hulls, 10-15% rice bran, 15-20% bran, 5% corn flour, and 64-66% water content.
[0029] 3) Obtaining spores: Parents undergo ac...
Embodiment 2
[0039] Embodiment 2 bacterial age test
[0040] In this embodiment, the new strain Finc-B-1 of the present invention is compared with the existing TNN-11 and Hakata A-264 to illustrate the new strain Finc-B-1 of the present invention. Advantages and features of B-1.
[0041] The cultivar culture medium that present embodiment adopts is:
[0042] 40-50% corn cob, 20-30% miscellaneous sawdust, 10-15% rice bran, 15-20% bran, 5% corn flour, of which the water content is 64-66%.
[0043] Taking TNN-11 as a control, set 6 bacterial ages from 70 days to 100 days, every 5 days is a bacterial age, and each bacterial age is 64 bottles.
[0044] Cultivation and cultivation environment conditions:
[0045] The culture temperature is 21~25℃, the humidity is 75%, CO 2 The concentration is 2500-4000ppm. The temperature of the cultivation room is set at 13-15°C, the humidity is 90-95%, and the CO 2The concentration is 1000-2000ppm, the light intensity is 50-100Lux in the first 1-7 days,...
Embodiment 3
[0049] Embodiment 3 consistency, stability test
[0050] This example tests the consistency and stability of Finc-B-1 when planted in different medium formulations A and B. in,
[0051] Medium A is:
[0052] 40-50% corn cob, 20-30% miscellaneous sawdust, 10-15% rice bran, 15-20% bran, 5% corn flour, of which the water content is 64-66%.
[0053] Medium B is:
[0054] Corn cob 20-30%, miscellaneous sawdust 20-30%, soybean hulls 20-30%, rice bran 10-15%, bran 15-20%, corn flour 5%, of which the water content is 64-66%.
[0055] The volume of the cultivation bottle is: 850cc.
[0056] Taking TNN-11 as a control, the cultivation effect of Finc-B-1 strain was tested. In this experiment, 6 small-scale cultivations (300 bottles / time) were designed, and the numbers were experiments 1, 2, 3, 4, 5 and 6. Among them, the medium formula used in experiments 1, 2 and 3 was formula A, and the experiment 4, The medium formula used in 5 and 6 is formula B. The parameters of cultivation ...
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