Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extract of effective components of antineoplastic gallnut as well as preparation method and application thereof

A technology of effective components and anti-tumor drugs, applied in the direction of anti-tumor drugs, drug combinations, medical raw materials derived from arthropods, etc., to achieve the effect of increasing added value and expanding uses

Active Publication Date: 2013-03-27
JIANGSU UNIV
View PDF11 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Chinese patent application CN200710041748.6 "A method for extracting tannin from Galla chinensis" relates to the extraction method of tannin from galla chinensis, but does not involve ether extraction components

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extract of effective components of antineoplastic gallnut as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of Galla gall anti-tumor effective component extract

[0026] The medicinal material Galla chinensis is dried and pulverized. Take 400g of medicinal powder, add 250ml of petroleum ether, stir evenly for 5min, let it stand for 1h, filter to remove petroleum ether, repeat 3 times, take out the medicine and dry it. Divide the above sample into 4 parts, add 1L of water, reflux extraction for 2 hours, repeat the extraction of medicinal dregs 4 times, and collect the filtrate. Concentrate the filtrate to about 200ml with a rotary evaporator, add 450ml of 95% ethanol, stir, and let stand overnight. Filter, take the filtrate, and use a rotary evaporator to evaporate the ethanol until there is no ethanol smell to obtain 200ml of crude extract. The crude extract was extracted four times with ether (250 ml of ether each time), the ether layers were combined, and evaporated with a rotary evaporator until there was no ether smell, and a brown extract was o...

Embodiment 2

[0028] Example 2: Preparation of Galla gall anti-tumor effective component extract

[0029] The medicinal material Galla chinensis is dried and pulverized. Take 400g of medicinal powder, add 350ml of petroleum ether, let it stand overnight, filter to remove petroleum ether, take out the medicine and dry it in the air. Divide the above sample into 4 parts, add 1 L of water, reflux extraction for 3 hours, repeat the extraction of medicinal dregs twice, and collect the filtrate. Concentrate the filtrate to about 300ml with a rotary evaporator, add 600ml of 95% ethanol, stir, and let stand overnight. Filter, take the filtrate, and use a rotary evaporator to evaporate the ethanol until there is no ethanol smell to obtain 300ml of crude extract. The crude extract was extracted three times with the same volume of ether, the ether layers were combined, and evaporated with a rotary evaporator until there was no ether smell, and a brown extract was obtained, which was the antitumor ...

Embodiment 3

[0031] Example 3: Preparation of Solid Galla Galla Antitumor Active Component Extract

[0032] The medicinal material Galla chinensis is dried and pulverized. Take 80g of medicinal powder, add 80ml of petroleum ether, stir evenly, let stand overnight, filter to remove petroleum ether, take out the medicine and dry it. The above sample was added to 600ml of water, refluxed for 2 hours, and the dregs were extracted twice, and the filtrate was collected. Concentrate the filtrate to about 80ml with a rotary evaporator, add 95% ethanol at a ratio of 95% ethanol:medicine solution 6:1, stir for 10 minutes, let stand overnight, filter, take the filtrate, and steam the ethanol out with a rotary evaporator. Until there is no ethanol smell, 80ml of crude extract is obtained. Extract the crude extract with 100ml of ether, extract twice, about 10 min each time, take the ether layers and combine them, evaporate them with a rotary evaporator until there is no ether smell, and dry them to o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an extract of effective components of antineoplastic gallnut. The extract is prepared from the following steps in sequence: drying the gallnut: crushing the dried gallnut; adding petroleum ether to remove the oil; drying in the air; carrying out water extraction and alcohol precipitation; evaporating to remove ethanol from the filtrate; extracting by diethyl ether; and concentrating the extractive liquid under diethyl ether, thus obtaining the extract of the effective components of antineoplastic gallnut. As the study shown, the extract of the effective components has the activity of inhibiting the growth of U251 (glioma cells) outside the body, so that the extract can be applied to preparation and development of antineoplastic medicines. The invention also discloses a preparation method of the extract.

Description

technical field [0001] The present invention relates to the extract of the anti-tumor effective part of Galla chinensis, the pharmaceutical composition containing it, its preparation method and application. Background technique [0002] Tumor is one of the most serious diseases that endanger human health in the world today. At present, tumor treatment includes surgery, radiotherapy, chemotherapy, intervention and other means, but the mortality rate is still high, and has now risen to the first [see: Xiao Zhenyu, He Shaozhong, Peng Weiwei. 2011, 22(23): 2204-2206]. Experts and scholars from all over the world are actively researching methods and drugs for treating tumors. Traditional Chinese medicine has a long history of treating difficult diseases such as cancer, and the active ingredients in medicinal plants can exert their biological effects through multiple channels and multiple targets. In particular, plant-derived drugs and their derivatives, such as vincristine, hyd...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00
Inventor 余江南童姗姗徐希明付敏杨艳冯颖淑
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com