Method for preparing soybean peptides from carrier-free immobilized enzyme

Abstract

A method for preparing soybean peptides from carrier-free immobilized enzyme belongs to an extract technology of soybean peptides. The method comprises the following steps of: (1) dissolving alkaline protease into phosphate buffer, adding ethanol to precipitate and gather, and then adding glutaraldehyde to carry out crosslinking, so as to obtain cross-linked enzyme aggregate, namely immobilized alkaline protease; and (2) adding water into isolated soy protein solution to be mixed and prepared into the isolated soy protein solution, adjusting the pH and the temperature, and then adding the immobilized enzyme obtained from the step (1) to carry out enzymolysis, recovering the immobilized enzyme after enzymolysis, centrifugally separating the enzymatic hydrolysate to obtain clear solution, and drying the clear solution to obtain the soybean peptides by freezing and drying. The carrier-free immobilized alkaline protease prepared by the method is high in activity, and does not need a high-cost carrier, and meanwhile, the isolated soy protein is subjected to enzymolysis by the immobilized enzyme to prepare the soybean peptides; the method is simple in required processing equipment, and low in energy consumption; and the immobilized enzyme can be recycled and reused. Thus, waste of resources is reduced, and the cost is saved.

Description

technical field [0001] The invention belongs to soybean polypeptide extraction technology, in particular to a method for preparing soybean polypeptide without carrier immobilized enzyme. Background technique [0002] Immobilized enzyme is a new technology developed in the 1960s. Generally, the enzyme is combined with an insoluble carrier, so that the enzyme is in a closed state in a certain space and can react continuously. After the reaction, the enzyme is passed through filtration or centrifugation, etc. The method is recycled and reused. As a main research direction in the field of enzyme application, immobilized enzymes are currently mostly immobilized on insoluble polymers or inorganic carriers, and the resulting immobilized enzymes are greatly reduced due to the presence of polymer carriers in the conversion of macromolecular substrates. The binding capacity and reactivity of the enzyme. In addition, the cost of reagents and carriers used for immobilization is high, ...

AI Technical Summary

Problems solved by technology

However, the application of cross-linked enzyme aggregate technology-carrier-free immobilized enzyme technology to the enzymatic hydrolysis of soybean protein isolates to extract soybean polypeptides has not been reported. The existing methods for extracting soybean polypeptides have problems such as that the enzymes cannot be recycled and reused, and the cost is high.

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