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Method for fermenting butanol and co-producing dietary fiber by corn bran

A technology of dietary fiber and butanol fermentation, which is applied in the direction of fermentation, can solve the problems of high cost, achieve low cost, realize waste utilization, and increase the added value of products

Active Publication Date: 2014-07-23
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the remaining cellulose is also degraded into simple sugars and then used for fermentation, it will require a high cost

Method used

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  • Method for fermenting butanol and co-producing dietary fiber by corn bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Weigh 5 g of corn bran that has been pulverized by ultra-fine airflow to a particle size of 200 mesh, add 50 mL of 0.5% (w / w) sulfuric acid solution (the solid-liquid ratio of corn bran flour to dilute sulfuric acid is 1:10), and react at 140 ° C for 20 min , adjust the pH value of the hydrolyzate to 4.8, add 200 IU / g substrate of glucoamylase, 1.0 IU / g substrate of xylanase, enzymatically hydrolyze at 50°C for 48h, and the speed of the shaker is 150r / min. Filtrate the enzymatic solution, the solid residue is dietary fiber, the dry weight is 1.75g, the swelling property is 1.83g / mL, and the water holding capacity is 195.7%; take the enzymatic solution and add 1% (w / v) activated carbon to detoxify at 50°C for 1 hour After filtering off the activated carbon, add 0.5% (w / v) corn steep liquor powder to the supernatant, inoculate NCIMB8052 strain, and incubate anaerobically at 37°C for 48h. The components of the fermentation product were analyzed by gas chromatography: butan...

Embodiment 2

[0038] Weigh 5g of corn bran that has been pulverized by ultra-fine airflow to a particle size of 100 mesh, add 40mL of 1% (w / w) sulfuric acid solution, and react at 150°C for 20min (the solid-liquid ratio of corn bran flour to dilute sulfuric acid is 1:8) , adjust the pH value of the hydrolyzate to 5.0, add 250 IU / g substrate of glucoamylase, 0.8 IU / g substrate of xylanase, enzymatically hydrolyze at 50°C for 48 hours, and the speed of the shaker is 150r / min. Filtrate the enzymolysis solution, the solid residue is dietary fiber, the dry weight is 1.86g, the swelling property is 1.71g / mL, and the water holding capacity is 191.5%; take the enzymolysis solution and add 2% (w / v) activated carbon After 0.5h of poisoning, filter out the activated carbon, add 0.3% (w / v) ammonium sulfate to the supernatant, inoculate NCIMB8052 strain, and incubate anaerobically at 37°C for 48h. Gas chromatography analysis product composition is: butanol 8.64g / L, total solvent 10.38g / L.

Embodiment 3

[0040]Weigh 5 g of corn bran that has been generally crushed to a particle size of 10 mesh, add 60 mL of 1.5% (w / w) sulfuric acid solution, and react at 120 ° C for 30 minutes (the solid-liquid ratio of corn bran flour to dilute sulfuric acid is 1:12), adjust The pH value of the hydrolyzate was 4.8, 300 IU / g substrate of glucoamylase and 1.0 IU / g substrate of xylanase were added, enzymolysis was carried out at 50°C for 48 hours, and the rotation speed of the shaker was 150r / min. Filtrate the enzymolysis solution, the solid residue is dietary fiber, the dry weight is 1.91g, the swelling property is 1.55g / mL, and the water holding capacity is 182.6%; take the enzymolysis solution and add 0.5% (w / v) activated carbon After poisoning for 1 hour, filter out the activated carbon, add 0.3% (w / v) corn steep liquor to the supernatant, inoculate NCIMB8052 strain, and incubate anaerobically at 37°C for 48 hours. The product components were analyzed by gas chromatography of the fermentatio...

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Abstract

The invention relates to the field of biological energy, and in particular relates to a method for fermenting butanol and co-producing dietary fiber by corn bran, which comprises the following concrete steps of: 1) pre-processing; 2) enzymatically hydrolyzing; 3) implementing solid-liquid separation, to obtain dietary fiber and enzymatic hydrolysate; 4) detoxifying the enzymatic hydrolysate; 5) refilling a nitrogen source into the detoxified enzymatic hydrolysate to prepare a fermenting culture medium; fermenting and breeding butanol by the sterilized fermenting culture medium under an anaerobic condition to produce a strain, so as to obtain a fermented product; and 6) rectifying the fermented product and collecting the butanol. According to the method for fermenting the butanol and co-producing the dietary fiber by the corn bran, measures are adjusted to local conditions, and the corn bran which is a byproduct of processing the corn is adopted as a raw material to ferment the butanol, so not only the waste is utilized to bring about benefit for environmental protection but the product additional value is improved for deep processing of the corn; and a way of improving the economic benefit is provided for the enterprises.

Description

technical field [0001] The invention relates to the field of bioenergy, in particular to a method for producing butanol and co-producing dietary fiber by fermenting corn hulls. Background technique [0002] Butanol is an important C4 platform compound, mainly used in the synthesis of n-butyl phthalate, butyl phosphate, butyl acrylate and butyl acetate, etc. It can also be used as an extraction solvent for oils, medicines and spices and Alkyd resin additives and other fields. With the diversified development of biomass energy, butanol is considered to be one of the most promising new liquid fuels because of its high energy density, low hydrophilicity, low vapor pressure, and high octane number. [0003] The traditional fermentation method to produce butanol uses grain raw materials (such as corn, wheat, etc.) The market competitiveness of alcohol products has restricted the promotion and use of bio-butanol as fuel, and is also contrary to my country's food security strategy....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16
Inventor 刘莉史吉平林增祥张丽丽姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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