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Method for producing cordycepin through semi-continuous liquid fermentation of cordyceps militaris

A technology for liquid fermentation and Cordyceps militaris, applied in directions such as fermentation and chemical recovery, can solve the problems of short utilization time of thalli, insufficient utilization of Cordyceps militaris cells, low fermentation intensity, etc., and achieves easy automatic production, target yield and The effect of high conversion rate and high fermentation intensity

Inactive Publication Date: 2013-03-13
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported method of producing cordycepin by liquid fermentation is mainly a batch fermentation process. Cordycepin mainly exists in the fermentation liquid, and the fermentation period is 10-15 days. Short body utilization time and low fermentation intensity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Strain activation:

[0030] Use an inoculation loop to take Cordyceps militaris ( Cordyceps militaris ) mycelium was inoculated on PDA solid medium, cultured in the dark at 25°C for 5 days, the surface of the medium was covered with mycelia, and then transferred to the condition of light, cultured for 5 days, all the mycelia changed from white to orange, and obtained Activated lawn;

[0031] 2. Preparation of seed solution:

[0032] Take an area of ​​6 ~ 10mm 2 Two pieces of bacterial lawn were inserted into the seed medium (100mL medium in a 250mL Erlenmeyer flask) and cultured for 5 days at 25°C with a shaker speed of 150r / min to obtain seed liquid. Mycelium balls have regular shape and uniform particles;

[0033] The preparation method of seed medium: potato juice 200mL, yeast powder 10g, glucose 40g, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0 g and magnesium sulfate (MgSO 4 ) 1.0 g was dissolved in 1000 mL tap water, mixed evenly, the pH was natural, ...

Embodiment 2

[0045] 1. Strain activation:

[0046] Use an inoculation loop to take Cordyceps militaris ( Cordyceps militaris ) mycelia were inoculated on PDA solid medium, cultured in the dark at 25°C for 5 days, the surface of the medium was covered with mycelia, and then transferred to the condition of light, and cultured for 5 days, all the mycelia changed from white to orange, and obtained Activated lawn;

[0047] 2. Preparation of seed solution:

[0048] Take an area of ​​6 ~ 10mm 2 Two pieces of bacterial lawn were inserted into the seed medium (100mL medium in a 250mL Erlenmeyer flask) and cultivated for 5 days at 25°C with a shaker speed of 150r / min to obtain seed liquid. Mycelium balls have regular shape and uniform particles;

[0049] The preparation method of seed medium: potato juice 200mL, yeast powder 10g, glucose 40g, potassium dihydrogen phosphate (KH 2 PO 4 ) 2.0 g and magnesium sulfate (MgSO 4 ) 1.0 g was dissolved in 1000 mL tap water, mixed evenly, the pH was nat...

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Abstract

The invention discloses a method for producing cordycepin through semi-continuous liquid fermentation of cordyceps militaris, and the method comprises the following concrete operating steps of: (1) putting hyphae of the Cordyceps militaris on a PDA (Potato Dextrose Agar) solid culture medium and activating to generate a bacterial lawn with a plenty of dense mycelia; (2) putting the bacterial lawn on a seed culture medium, carrying out shake cultivation, and forming a plenty of mycelium pellets after 5 days, thereby obtaining seed liquid; (3) transferring the seed liquid into a seed liquid fermentation tank to carry out expanding culture; (4) transferring the seed liquid after being subjected into the expanding culture in a 500L tank to carry out first-batch fermentation; and (5) carrying out semi-continuous fermentation in 10 batches in the 500L tank under the same conditions. In the fermentation process, a method of adjusting the culture medium composition and the fermentation conditions is adopted to control the cordyceps militaris to form the mycelium pellets with regular shape and uniform size and the average diameter of the mycelium pellets is 3-5mm, so that the separation of fermentation broth and thalli is easy; the carbon and nitrogen source and other inorganic salts needed in the growth of mycelium pellets are saved by utilizing the repeated fermentation of the mycelium pellets of the cordyceps militaris, so that the cost is saved; and the fermentation cycle is shortened and the fermentation intensity is increased, so that the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbes and fermentation engineering, and specifically relates to a method for producing cordycepin by semi-continuous liquid fermentation of Cordyceps militaris by using mycelium balls formed by the aggregation and flocculation of Cordyceps militaris mycelia in a culture system. Background technique [0002] Cordyceps militaris ( Cordyceps militaris ), an important species of the genus Cordyceps, is a strong pathogenic fungus for insects, containing various active ingredients such as cordycepin, cordyceps polysaccharide, and cordycepic acid. Among them, cordycepin (cordycepin, 3'-deoxyadenosine) is a nucleoside antibiotic. It has a variety of biological activities and has been proven to improve human immunity. It has shown certain resistance to tumors and pathogenic bacteria and fungi, and has become a research hotspot in recent years. It has been successfully used to treat human African in mouse model...

Claims

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Application Information

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IPC IPC(8): C12P19/40
CPCY02P20/582
Inventor 魏兆军方婷婷章建国
Owner HEFEI UNIV OF TECH
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