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Saccharomyces cerevisiae strain and application of strain in production of ethanol by co-fermentation of glucose and xylose

A Saccharomyces cerevisiae strain and co-fermentation technology, applied in the field of bioengineering, can solve the problems of slow xylose metabolism rate, affecting ethanol yield and economy, etc.

Active Publication Date: 2013-03-13
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the xylose isomerase pathway has its own problems: xylose is metabolized at a slow rate, affecting the yield and economy of ethanol in the process

Method used

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  • Saccharomyces cerevisiae strain and application of strain in production of ethanol by co-fermentation of glucose and xylose
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  • Saccharomyces cerevisiae strain and application of strain in production of ethanol by co-fermentation of glucose and xylose

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Experimental program
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Effect test

Embodiment 1

[0020] Construction and Screening of Strain SyBE003

[0021] Departure strain: Departure strain L2612 was donated by Professor Thomas Jeffries of the University of Wisconsin-Madison. The genotype is MAT alpha, leu2, ura3, trp1. It is deficient in leucine, uracil and tryptophan.

[0022] culture medium

[0023] Screening medium A: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L (refer to [US] D.C. Amberg et al. Yeast Genetics Method Test Guide for specific formula), leucine 100mg / L, tryptophan 100mg / L, glucose 20g / L.

[0024] Screening medium B: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, tryptophan 100mg / L, glucose 20g / L.

[0025] Screening medium C: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, glucose 20g / L.

[0026] Screening medium D: synthetic nitrogen source YNB 6.7g / L, mixed amino acid powder 2g / L, aureobasicin A 0.5mg / L, glucose 20g / L.

[0027] Acclimatization medium: synthetic nitrogen source YNB 6....

Embodiment 2

[0038] Comparing the fermentation performance of the strain SyBE003 and the starting recombinant strain SyBE002 on the glucose / xylose medium (20g / L each)

[0039] 1. Test materials: strain SyBE003 and starting strain SyBE002.

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Abstract

The invention discloses a saccharomyces cerevisiae strain and application of the strain in production of ethanol by co-fermentation of glucose and xylose. The saccharomyces cerevisiae strain is named as SyBE003 and preserved in the China General Microbiological Culture Collection Centre, and has a preservation number of CGMCC No. 6633 and a capacity of producing ethanol by co-fermentation of glucose and xylose. The saccharomyces cerevisiae strain disclosed by the invention can effectively jointly utilize glucose and xylose for fermentation to produce ethanol. The utilization rates of the saccharomyces cerevisiae strain for both glucose and xylose achieve 100%, the yield of ethanol achieves 80% of an ideal yield, the yield of a by-product, namely, xylitol, is less than 2%, and pentose and hexose can be efficiently converted, and therefore, the saccharomyces cerevisiae strain is an excellent strain for utilizing cellulose hydrolysate.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Saccharomyces cerevisiae strain capable of co-fermenting glucose and xylose and its application. Background technique [0002] my country's demand for energy is increasing day by day, but the shortage of fossil resources such as oil is becoming more and more serious, which greatly restricts the rapid and healthy development of my country's national economy. At the same time, the burning of fossil resources has caused serious damage to the ecological environment. Seeking renewable and clean energy that can replace or partially replace fossil resources is a major scientific research challenge faced by Chinese scientific and technological workers. [0003] Fuel ethanol is considered to be one of the most promising clean bioenergy sources. However, the development of fuel ethanol using grain as raw material is contrary to the basic national policy of ensuring fo...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 元英进査健胡梦龙申明华
Owner TIANJIN UNIV
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