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Method for extracting buckwheat flavone from buckwheat bran

A technology of buckwheat bran and buckwheat flavonoids, which can be used in pharmaceutical formulas, non-central analgesics, medical preparations containing active ingredients, etc., and can solve the problems of low yield and content of total flavonoids, large energy consumption, and solvent residues , to achieve the effect of simple and easy operation, energy saving and fast completion time

Inactive Publication Date: 2013-03-06
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the excessive energy consumption of these methods, low yield and content of total flavonoids, many impurities, and solvent residues, some emerging extraction techniques have received more and more attention, such as ultrasonic-assisted extraction and microwave heating. , semi-bionic method, enzyme-assisted extraction method, etc.

Method used

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  • Method for extracting buckwheat flavone from buckwheat bran
  • Method for extracting buckwheat flavone from buckwheat bran
  • Method for extracting buckwheat flavone from buckwheat bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for extracting buckwheat flavonoids from buckwheat bran comprises the following steps:

[0034] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as the raw material, and the crushing method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set aside;

[0035] Enzymolysis under ultrasonic conditions: take the pretreated buckwheat bran, add 45 times the amount of water to make a buckwheat bran suspension, adjust the pH value to 4.6, add enzyme to buckwheat bran according to the amount of 0.54mg / ml Add the extraction compound enzyme (purchased from Ningxia Hersbit Biotechnology Co., Ltd., including cellulase, β-glucanase, xylanase, pectinase and acid protease) to the suspension, mix well, and The enzymolysis was carried out for 71 minutes at a temperature of 40° C. and a power of 200 W to obtain an enzymolysis solution.

[0036] (2) Centrifugal separation: centrifuge the enzymat...

Embodiment 2

[0052] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as raw material, and the pulverization method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set it aside; the specific steps of extracting buckwheat bran flavonoids are as follows:

[0053] Enzymolysis under ultrasonic conditions: Take crushed buckwheat bran, add 50 times the amount of water to make buckwheat bran suspension, adjust the pH value to 4.5, and add enzyme amount of 0.50mg / ml to buckwheat bran to suspend Add cellulase (enzyme activity ≥ 400,000 u / g), pectinase (enzyme activity ≥ 500,000 u / g) and acid protease (enzyme activity ≥ 100,000 u / g) into the turbid liquid and mix well, three of them The mass ratio of the enzymes was 1:1:1; the enzymolysis was carried out for 80 min under the conditions of an ultrasonic temperature of 40°C and a power of 200W to obtain an enzymatic hydrolysis solution.

[0054] (2) Centrifugal separa...

Embodiment 3

[0060] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as raw material, and the pulverization method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set it aside; the specific steps of extracting buckwheat bran flavonoids are as follows:

[0061] Enzyme hydrolysis under ultrasonic conditions: Take crushed buckwheat bran, add 40 times the amount of water to make buckwheat bran suspension, adjust the pH value to 4.0, and add enzyme amount of 0.70mg / ml to buckwheat bran to suspend Add the extraction compound enzyme to the turbid solution and mix well, and perform enzymolysis for 40 minutes under the condition of ultrasonic temperature of 40°C and power of 200W to obtain an enzymolysis solution.

[0062] (2) Centrifugal separation: centrifuge the enzymatic hydrolyzate prepared in step (1) at 3500 rpm for 15 minutes, and collect the precipitate and supernatant.

[0063] (3) Ultrasonic alcohol ext...

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Abstract

The invention discloses a method for extracting buckwheat flavone from buckwheat bran. A method of combining enzyme treatment and ultrasonic assistance is adopted for extracting the buckwheat flavone from the buckwheat bran, and in addition, macroporous resin is adopted for purification. The method comprises the following steps of 1) carrying out enzymolysis under the ultrasonic condition: the buckwheat bran is taken and is added with water to be prepared into buckwheat bran turbid liquid, and then, compound enzyme is added for carrying out enzymolysis; 2) centrifugal separation: enzymatic hydrolysate is subjected to centrifugation for collecting precipitates and supernate; 3) ultrasonic alcohol extraction: precipitates are subjected to ultrasonic leaching by ethanol; 4) filtration and concentration: alcohol extraction liquid is filtered and concentrated; 5) purification: the macroporous resin is used for carrying out purification on the concentrated liquid; and 6) drying: buckwheat-flavone-containing fractions obtained through purification are subjected to vacuum drying, and flavone products are obtained. The flavone compound prepared by the method disclosed by the invention has the advantages that the purity is higher, the preparation process is simple, the operation is simple and convenient, the implementation is easy, the environment is friendly, and the flavone compound is suitable for industrial magnification production.

Description

technical field [0001] The invention belongs to the field of food processing, in particular to a method for extracting buckwheat flavonoids from buckwheat bran. Background technique [0002] Buckwheat, also known as triangular wheat and rye, is a short-season Polygonaceae plant that grows in a low temperature environment. Botanically, it mainly includes two cultivated varieties of sweet buckwheat and tartary buckwheat, which are ideal crops for supplementary crops in food crops. Buckwheat is rich in nutrition and is a food crop with both medicine and food. Studies have found that buckwheat bran contains more flavonoids. Flavonoids have strong physiological activity, and their main functions are as follows: regulate myocardial contraction, improve myocardial relaxation, and have a good preventive effect on myocardial ischemia, myocardial infarction, and arrhythmia; remove free radicals in the body, directly Inhibit the growth of cancer cells, resist carcinogenic factors, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/30A61K36/70A61P9/04A61P9/10A61P9/06A61P39/06A61P35/00A61P3/10A61P3/06A61P5/38A61P31/04A61P29/00
Inventor 杨芙莲陈旭清何琳
Owner SHAANXI UNIV OF SCI & TECH
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