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Method of diagnostic of obesity

An obesity and genetic technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as the inability to cultivate intestinal symbionts

Inactive Publication Date: 2013-02-20
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
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  • Abstract
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Problems solved by technology

[0005] Most intestinal symbionts cannot be cultured

Method used

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  • Method of diagnostic of obesity
  • Method of diagnostic of obesity
  • Method of diagnostic of obesity

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Embodiment Construction

[0020] method

[0021] Human stool sample collection. Danish individuals from the Inter-99 population (Toft. et al., Prev. Med., 47:378-383, 2008) differed phenotypically according to BMI (Body / Mass Index) and status towards obesity / diabetes. Patients and healthy controls were asked to provide frozen stool samples. Obtain fresh stool samples at home and immediately freeze the samples by storing them in their home freezer. Frozen samples were delivered to the hospital using closed polystyrene foam containers, and they were then stored at -80°C until analysis.

[0022] DNA extraction. A frozen aliquot (200 mg) of each stool sample was suspended in 250 μl of guanidine isothiocyanate, 0.1 M Tris (pH 7.5) and 40 μl of 10% N-lauroyl sarcosine. DNA extraction was then performed as previously described (Manichanh et al., Gut, 55:205211, 2006). The concentration of DNA and its molecular size were estimated by NanoDrop (Thermo Scientific) and agarose gel electrophoresis.

[0023] ...

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Abstract

A new method for diagnosing obesity is herein described, based on the determination of the absence of at least one gene from the human' gut microbiome.

Description

technical field [0001] The human gut microbiota (microbiota) constitutes a complex ecosystem whose impact on human health and well-being is now well recognized. This indeed contributes to the maturation of the immune system and the immediate barrier to pathogen colonization. In the last half century, infectious diseases have been significantly reduced and major pathogens have been brought under control. During the same period, the prevalence (prevalence) of some "immune" diseases continued to increase, especially in Western societies. This is the case for allergies, inflammatory bowel disease, irritable bowel syndrome and possibly metabolic and degenerative diseases such as obesity, metabolic syndrome, diabetes and cancer. The sequence of the human genome has led to the observation of genes associated with increased risk of immune disease, but mutations in most of these genes usually explain only a small part of what is actually going on, and genetic predispositions would re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/689C12Q2600/158C12Q1/6883
Inventor S·埃利克
Owner INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
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