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Method for preparing extracts rich in vitexin rhamnoside and vitexin glucoside

A technology for vitexin rhamnoside and vitexin glucoside is applied in the field of preparing high-content vitexin rhamnoside and vitexin glucoside extract, which can solve the problem of large usage of organic solvent, complicated preparation method, The problems of low content of vitexin rhamnoside and vitexin glucoside can achieve the effect of good impurity removal effect, high product purity and easy crystallization

Inactive Publication Date: 2013-02-13
苏州衷中医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems in the prior art that the contents of vitexin rhamnoside and vitexin glucoside in hawthorn leaf extracts are relatively low, the preparation method is complicated, and the amount of organic solvent used is large, the invention provides a method for preparing vitexin-rich rat The method of glucoside and vitexin glucoside extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 1 kg of hawthorn leaves, add water to decoct 2 times, add 10 times the amount of water for the first time, and 6 times the amount of water for the second time, each time for 1 hour, after the obtained extract is filtered, the aqueous solution of the crude extract of hawthorn leaves is obtained. For amide column adsorption, first wash with 10 times the column volume of water to remove impurities. Impurities were then eluted sequentially with 5%, 10% and 20% ethanol aqueous solutions, and each time the volume was 5 times the column volume. Then the target component was eluted with 10% ethanol aqueous solution containing 10g / L sodium bicarbonate, the amount of eluent was 10 times the column volume, and the eluate was collected.

[0030] The pH of the eluent was adjusted to 7 with hydrochloric acid, the ethanol was recovered until it had no alcohol smell, and 500 ml of the eluent without organic solvent was obtained.

[0031] The organic solvent-free eluate was extrac...

Embodiment 2

[0033]Take 1 kg of hawthorn leaves, add 60% ethanol aqueous solution to reflux extraction twice, add 8 times the amount for the first time, and 6 times the amount for the second time, each time for 1 hour, filter the obtained extract, recover the ethanol until it has no alcohol smell, and dilute with water to obtain The aqueous solution of the crude extract of hawthorn leaves was adsorbed on a D101 macroporous adsorption resin column, and first washed with 10 times the column volume of water to remove impurities. Then use 5%, 15%, and 30% ethanol aqueous solution to elute impurities in sequence, and the amount of ethanol aqueous solution each time is 5 times the column volume. Then the target component was eluted with 15% ethanol aqueous solution containing 5g / L potassium bicarbonate, the amount of eluent was 8 times the volume of the column, and the eluate was collected.

[0034] Adjust the pH of the eluent to 6.5 with sulfuric acid, recover ethanol until it has no alcohol sm...

Embodiment 3

[0037] Take 200g of the commercially available water extract of hawthorn leaves (tested by high performance liquid chromatography, the content of vitexin rhamnoside is 1.6%, and the content of vitexin glucoside is 0.7%), dissolve in 2000ml water, filter, and make clarified hawthorn leaves The aqueous solution of the crude extract is adsorbed on an AB-8 macroporous adsorption resin column, and first washed with 10 times the volume of the column to remove impurities. To prepare a mixed solvent, mix n-propanol and ethanol at a volume ratio of 1:1. Use 10 times column volume of 5% mixed solvent aqueous solution and 5 times column volume of 30% mixed solvent aqueous solution to elute impurities in sequence. Then the target component was eluted with an aqueous solution of 20% mixed solvent containing 5g / L sodium bicarbonate, the amount of eluent was 8 times the volume of the column, and the alkaline eluent was collected.

[0038] The pH of the eluent was adjusted to 7 with hydrochl...

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Abstract

The invention provides a method for preparing extracts rich in vitexin rhamnoside and vitexin glucoside, and relates to the field of herbal extracting. The method comprises the following steps: a water solution of Chinese hawthorn crude extract is used for being passed through macroporous adsorption resin columns or polyamide columns, and water, solvents, solvents A containing sodium bicarbonate or potassium bicarbonate are used to elute target composition, and eluting liquid is collected. The potential of hydrogen (pH) is adjusted to be between 6 and 7.5, organic solvents are removed, eluting liquid free of the organic solvents is obtained, normal butanol or isobutyl alcohol is used for extracting the target composition, and the solvents are recovered to dry. Or the water solution of the Chinese hawthorn crude extract is used for being passed through the macroporous adsorption resin columns or the polyamide columns, salt is eluted by the water, and then solvents B are used to elute the target composition, the eluting liquid is recovered, and the extracts rich in the vitexin rhamnoside and the vitexin glucoside are obtained after the eluting liquid is dried. According to the method, the vitexin rhamnoside is 30%-55% of the extracts, and the vitexin glucoside is 10%-35% of the extracts.

Description

technical field [0001] The invention relates to the field of extracting Chinese herbal medicines, and more specifically relates to a method for preparing high-content vitexin rhamnoside and vitexin glucoside extracts using hawthorn leaf extracts as raw materials. Background technique [0002] Hawthorn leaves are rich in flavonoid active ingredients, which have extensive and exact therapeutic effects on cardiovascular and cerebrovascular diseases. The content of vitexin rhamnoside and vitexin glucoside in the hawthorn leaf extract is relatively high, and the structure and polarity of the two are similar, and they are the exclusive pharmacological active ingredients of the hawthorn leaf extract. The Chinese Pharmacopoeia 2010 Edition stipulates that vitexin rhamnoside and vitexin glucoside are used as the fingerprint characteristic index components of hawthorn leaf extract, and vitexin rhamnoside in the total flavonoid extract of hawthorn leaf is stipulated. Reach more than 8...

Claims

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Application Information

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IPC IPC(8): A61K31/7048C07H1/08C07H17/04C07D407/04A61P9/00
Inventor 石荣火陈文星严燕青叶华
Owner 苏州衷中医药科技有限公司
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