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Preparation method of rice bran selenoprotein powder

A protein powder and rice bran technology, which is applied in the field of functional food processing and selenium-enriched rice by-product processing, can solve the problems of high process cost and achieve high acceptability, avoid waste, and high use value

Active Publication Date: 2013-02-13
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The alkaline method is simple and easy to implement, and the process cost is low; the reaction conditions for the enzymatic preparation of rice bran protein are mild, but compared with the alkaline method, the process cost is higher

Method used

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  • Preparation method of rice bran selenoprotein powder
  • Preparation method of rice bran selenoprotein powder
  • Preparation method of rice bran selenoprotein powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Raw material pretreatment: The same batch of selenium-enriched rice is dehulled with a rice huller and milled with a rice mill to obtain coarse rice bran, which is then ground with a pulverizer and passed through an 80-mesh sieve.

[0026] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:5, degrease twice on a magnetic stirrer, 3 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0027] (3) Removing polyphenols: Mix 65% ethanol with defatted rice bran powder (the ratio of solid to liquid is 1:4), stir on a magnetic stirrer for 20 minutes, and then centrifuge. This process is repeated 3 times. The precipitates after centrifugation were combined and air-dried overnight in a fume hood.

[0028] (4) Alkali extraction: the defatted rice bran powder was extracted twice with NaOH at a concentration of 0.08 mol / L, the ratio of solid to liquid was 1:10, the temperature was 40°C, and the time was 3 h, centrifuged, and the super...

Embodiment 2

[0033] (1) Raw material pretreatment: The same batch of selenium-enriched rice is dehulled with a rice huller and milled with a rice mill to obtain coarse rice bran, which is then ground with a pulverizer and passed through an 80-mesh sieve.

[0034] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:5, degrease twice on a magnetic stirrer, 3 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0035] (3) Removing polyphenols: Mix 65% ethanol with defatted rice bran powder (the ratio of solid to liquid is 1:4), stir on a magnetic stirrer for 20 minutes, and then centrifuge. This process is repeated 3 times. The precipitates after centrifugation were combined and air-dried overnight in a fume hood.

[0036] (4) Alkaline extraction: the defatted rice bran powder was extracted twice with NaOH at a concentration of 0.08mol / L, the ratio of solid to liquid was 1:15, the temperature was 35°C, and the time was 2h, centrifuged, and the super...

Embodiment 3

[0041] (1) Raw material pretreatment: The same batch of selenium-enriched rice is dehulled with a rice huller and milled with a rice mill to obtain coarse rice bran, which is then ground with a pulverizer and passed through an 80-mesh sieve.

[0042] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:5, degrease twice on a magnetic stirrer, 3 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0043] (3) Removing polyphenols: Mix 65% ethanol with defatted rice bran powder (the ratio of solid to liquid is 1:4), stir on a magnetic stirrer for 20 minutes, and then centrifuge. This process is repeated 3 times. The precipitates after centrifugation were combined and air-dried overnight in a fume hood.

[0044] (4) Alkali extraction: the defatted rice bran powder was extracted twice with NaOH at a concentration of 0.02 mol / L, the ratio of solid to liquid was 1:20, the temperature was 40°C, and the time was 2 h, centrifuged, and the super...

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Abstract

The invention relates to the field of selenium-rich rice byproducts, and in particular relates to a preparation method of rice bran selenoprotein powder. The method comprises the following steps of: grinding, screening and degreasing byproduct rice bran obtained after rice processing to prepare rough rice bran into degreased rice bran, mixing 60%-70% alcohol with the degreased rice bran in the material-liquid ratio of (1:3)-(1:5), stirring on a magnetic force mixing machine for a certain period of time, and centrifuging, wherein the processes are repeated for three times; and carrying out alkaline extraction with 0.02-0.1mol / L NaOH solution for 1-3 hours in the material-liquid ratio of (1:10)-(1:20) at the temperature of 30-40 DEG C, taking supernate after centrifuging, decolorizing with active carbon, adjusting an isoelectric point by utilizing a acetic acid-natrium aceticum buffer solution for acid deposition, then centrifuging, and freezing to dry after washing, thereby obtaining the rice bran selenoprotein powder with the protein extraction ratio of 60-80%, and the selenium content of 0.2-0.3mg / kg finally. The selenoprotein powder provided by the invention is natural in nutritional ingredient, is rich in trace element selenium, keeps the original nutrition ingredients in the rice bran selenoprotein powder effectively, and is suitably used as a food auxiliary material or a nutrition enhancer.

Description

1. Technical field [0001] The invention relates to the field of processing selenium-enriched rice by-products, in particular to a method for preparing rice bran selenoprotein powder, and belongs to the field of functional food processing. 2. Background technology [0002] Rice is one of the main crops in my country, and rice bran is an important by-product produced in the rice processing process. my country's annual output of rice bran is more than 10 million tons, and the available rice bran resources are quite abundant. At present, rice bran is mainly used as low-value-added feed. In fact, rice bran is the most potential high-value-added resource. Its protein content is 12% to 18%. Not only that, rice bran also contains dietary fiber, vitamins, oryzanol, Minerals, phytic acid, protease inhibitors and alpha-amylase inhibitors, etc. Rice bran protein is a plant-based protein with rich nutritional value and hypoallergenic characteristics. It is mainly albumin and globulin, f...

Claims

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Application Information

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IPC IPC(8): A23J1/12
Inventor 胡秋辉陈曦方勇胡兴锁吴建苏谢谋程冯儒杨文建马宁赵立艳鞠兴荣
Owner NANJING UNIV OF FINANCE & ECONOMICS
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