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Method for preparing micro-molecular hemepeptide from duck blood

A technology of small molecule blood peptide and duck blood, which is applied in the preparation method of peptide, chemical instruments and methods, peptide, etc., to achieve the effect of increasing added value

Inactive Publication Date: 2013-02-06
NANCHANG UNIV
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AI Technical Summary

Problems solved by technology

At present, there are no public reports in domestic literature on the comprehensive utilization of duck blood to produce multifunctional small molecule blood peptides

Method used

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Experimental program
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Embodiment Construction

[0017] Processing step of the present invention is:

[0018] (1) Anticoagulation and antioxidation treatment of duck blood: 2 g / L ascorbic acid was used as an antioxidant, and the addition amount was 0.05%-0.1% of the blood volume; and 4 g / L trisodium citrate was used as an anticoagulant, The added amount is 16.7% of the blood volume;

[0019] (2) Centrifugation: centrifuge at 4000r / min for 30min, the supernatant is plasma, and the centrifuged sediment is blood cells;

[0020] (3) Hemolysis and cleavage of blood cells: Part of the blood cells are hemolyzed by using the precipitate blood cells obtained in step (2) by centrifugation, i.e. -20°C and 90°C, and then homogenized at 120MPa dynamic ultra-high pressure micro-jet Under-machine treatment once for cell destruction to obtain hemoglobin;

[0021] (4) Stage enzymatic hydrolysis control: divided into two stages, the first stage is at pH 8.5, temperature 55°C, using 0.5% alkaline protease for 2.5h; the second stage is at pH ...

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Abstract

A method for preparing micro-molecular hemepeptide from duck blood includes that the duck blood which is a meat duck processing byproduct is used as a raw material, and an anti-coagulation and anti-oxidation technology, a high and low temperature changing alternation technology, a dynamic ultrahigh-pressure technology, a phased enzymatic hydrolysis technology, an enzymatic hydrolysis supernatant decoloration treatment and membrane fractionation technology, spray-drying technology and the like are integrated to develop the micro-molecular hemepeptide with physiological activity within a specific molecular weight range. The average peptide chain length of the micro-molecular hemepeptide prepared by the method ranges from 2.9 amino acid residues to 3.5 amino acid residues, the molecular weight distribution range of the micro-molecular hemepeptide is 1000-3000d, and the content of hemepeptide is higher than or equal to 35%. The method has the advantages that an additional value of the product is increased, duck blood resources are totally, effectively and comprehensively utilized, a preparation process is free of pollutant discharge, and an ecological benefit of zero environmental pollution can be achieved.

Description

technical field [0001] The invention relates to a method for comprehensively producing small-molecule blood peptides by using duck blood as a raw material, and belongs to the fields of agricultural by-product finishing technology and food nutrition. Background technique [0002] Livestock blood is a food with high nutritional value, known as "liquid meat", it is rich in protein and trace elements (such as iron), as well as appropriate amount of minerals, vitamins, hormones, enzymes and other physiological activities substance. Animal blood is divided into plasma and blood cells, wherein the protein content of plasma is about 25% of the total protein content of whole blood, and the protein content of blood cells is about 15%. According to data, the number of poultry slaughtered in my country exceeds 9 billion per year, and the annual stock and slaughter volume basically maintain a growth rate of 5-10%. Usually, a poultry is slaughtered, and about 0.1kg of blood can be co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 刘伟刘成梅杨水兵钟业俊吴孛徐慧
Owner NANCHANG UNIV
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