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Method for producing fuel ethanol by fermenting corncob processing residue

A technology of fuel ethanol and corn cob, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., capable of solving problems such as low ethanol concentration

Inactive Publication Date: 2013-02-06
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problem that the final ethanol concentration of the fermentation unit is low in the process of producing ethanol by fermentation of corncob processing residues, the present invention improves the concentration of xylose residue in the fermentation liquid by compounding cellulase, adding non-grain carbon sources, and synchronous saccharification and fermentation. ethanol concentration, reducing energy consumption in the ethanol concentration stage

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  • Method for producing fuel ethanol by fermenting corncob processing residue
  • Method for producing fuel ethanol by fermenting corncob processing residue

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Experimental program
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Embodiment 1

[0022] Take 100 g of corn cob processing residue (75% water content, 63% fiber content in the dry basis of corn cob processing residue), add appropriate amount of NaOH to adjust the pH to 5.0, add 6.3 g of compound enzyme preparation and 170 g of water, and put it in 1L glass for reaction In the tank, hydrolyze at 150 rpm and 50 ℃. Add 100 g of corncob processing residue at 3 h, 5 h and 21 h, respectively. After 27.5 hours, lower the temperature to 35°C, insert the cultured heat-resistant Saccharomyces cerevisiae FE-B, the amount of yeast introduced is 1‰ (the ratio of dry yeast mass to the system), and start to add molasses to the system after 28 hours (The total sugar concentration of molasses is 52.69%), the flow acceleration rate is 3.7 g / h, the feeding time is 28h-60h, and a total of 118.4 g molasses is added to the system. Results After 72 h, the ethanol concentration in the simultaneous saccharification and fermentation system of xylose residue was 9.7% v / v (concentrat...

Embodiment 2

[0024]The co-fermentation experiment of corncob processing residue and molasses was carried out in a 5L fermenter. Take 1000 g of corn cob processing residue (75% water content, 63% fiber content in dry basis of corn cob processing residue), add an appropriate amount of NaOH to adjust the pH to 5.0, add 100 g of compound enzyme preparation and 1700 g of water, and put it in 5 L for fermentation Into the tank, insert the cultured temperature-resistant Saccharomyces cerevisiae FE-B, the amount of yeast added is 1 wt‰ (the ratio of the dry yeast mass to the system), and the simultaneous saccharification and fermentation are carried out at 150 rpm and 42 °C. At 16 h and 24 h, 750 g and 250 g of corncob processing residue were added twice, respectively. At 0 h, molasses (total sugar concentration: 52.69 %) was added to the system, the flow acceleration rate was 3.7 g / h, and the feeding time was from 0 h to 96 h. A total of 996 g of molasses was added to the system. Results After 1...

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Abstract

The invention relates to a method for producing fuel ethanol by fermenting corncob processing residue. The method comprises the steps of (1) preparing an obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the compound enzyme preparation comprises endo cellulose, exo cellulose and beta-glucosidase; (2) adding the corncob processing residue to the obligate hydrolysis compound enzyme preparation for the corncob processing residue, wherein the content of the compound enzyme preparation is 0.05-0.5 g in per gram cellulose; pre-enzymolyzing for 0-36 h at a temperature of 45-60 DEG C; then adjusting the temperature to 24-44 DEG C; adding 0.002%-0.005% of saccharomyces cerevisiae; and performing simultaneous sacchrification and fermentation on the corncob processing residue to produce ethanol. Non-crop fermentation raw materials can be supplemented during the fermentation process. Compared with a conventional method, the method provided by the invention solves the problem of low final ethanol concentration of a fermentation unit during the process of producing ethanol by fermenting the corncob processing residue, and reduces energy consumption of an ethanol concentrating stage.

Description

technical field [0001] The invention relates to a method for producing fuel ethanol, in particular to a method for fermenting and producing fuel ethanol by using corncob processing residue as the main raw material. Background technique [0002] A large amount of xylose residue and furfural residue will be produced in the process of processing furfural and xylose with corn cob as raw material, collectively referred to as corn cob processing residue. my country discharges nearly 30 million tons of furfural waste residues every year (CN 200910090506.5). The stacking of these waste residues not only occupies land, but also pollutes the surrounding environment. It has been determined that the cellulose content in the dry basis of corncob processing residues accounts for more than 60%, about 8% of hemicellulose, and about 26% of lignin. CN00107969.7 discloses a method for producing ethanol by utilizing xylose residue. The patent first adopts Aspergillus niger solid fermentation t...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/16Y02E50/10
Inventor 张全黎元生佟明友金平乔凯
Owner CHINA PETROLEUM & CHEM CORP
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