Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological mosquitocide and preparation method thereof

A mosquito killer and biological technology, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of not obvious mosquito killing effect, slow mosquito killing process, unacceptable and difficult to achieve drug resistance , reduce the cost of mosquito control, and have a stable effect

Active Publication Date: 2013-02-06
郑文定 +1
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mosquito killing process of Pg is relatively slow, and the mosquito killing effect is not obvious when it is just applied, so it is not easy to be accepted by everyone, and its application is thus limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological mosquitocide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of a biological mosquito killer includes the following steps:

[0037] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, and crush with a crusher 10 seconds, standby;

[0038] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0039] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0040] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0041] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used: 1.4×10 5 , G...

Embodiment 2

[0048] A preparation method of a biological mosquito killer includes the following steps:

[0049] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker rotation speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, high speed grinder Crush for 10 seconds, standby;

[0050] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0051] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0052] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0053] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used:...

Embodiment 3

[0059] (1) Use SFE or KPYG2 liquid culture medium to culture Pg on a shaker for 5 days, culture temperature 25℃±1℃, shaker speed 120rpm; filter out the hyphae, wash with sterile water 3 times, add water to the original volume, and crush with a crusher 10 seconds, standby;

[0060] (2) Prepare 1%~3% (W / V) sodium alginate solution;

[0061] (3) Mix the Pg mycelium suspension with the sodium alginate solution in a ratio of 1:4, and mix well;

[0062] (4) Slowly drip the above-mentioned mixture into 1%~5%(W / V) calcium chloride solution, microcapsule particles are formed and precipitated. After 0.5~3 hours, the microcapsules are filtered out and rinsed with water for later use;

[0063] (5) Inoculate Bti bacteria in Bti liquid medium, culture on a shaker, rotate at 120rpm, temperature 30℃±1℃; after 48 hours, collect spores by centrifugation, and dilute to the following density when used: 1.4×10 9 , Get the spore suspension;

[0064] (6) Mix the spore suspension and the Pg microcapsules at t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a biological mosquitocide and a preparation method thereof. The biological mosquitocide is prepared by compounding mosquito-killing funguses and mosquito-killing bacteria; and on the basis of the mosquito-killing funguses in 1g of wet hypha, mosquito-killing funguses, i.e., bacillus thuringiensis subsp. israelensis with the number of 1.4*10<4>-1.4*10 <18> or mosquito-killing bacteria, i.e., bacilluss phaericu with the number of 2.5*10<4>-2.5*10<20> are provided. The mosquito-killing fungus is Pythium Guiyang nse Su or Pythium caronlinianum Matthews. The biological mosquitocide has the effects of killing mosquitoes fast and controlling mosquitoes in a lasting form.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biological mosquito killer and a preparation method of the biological mosquito killer. [0002] Background technique [0003] Agricultural pests and forestry pests bring serious losses to human agriculture and forestry production every year. At the same time, mosquitoes not only harass the crowd, but also can spread more than 20 kinds of diseases, threatening human health. Thanks to the invention of chemical pesticides, people have regained food from insects, controlled the number of vector insects, and reduced the prevalence of insect-borne diseases. [0004] However, the long-term and extensive use of chemical pesticides has caused serious problems. 1) Environmental pollution, which is harmful to human and biological health. Heavy metals in pesticides remain in the soil, causing soil deterioration and reduced agricultural production; agricultural products contain high levels ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/04A01N63/02A01P7/04
Inventor 苏晓庆
Owner 郑文定
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com