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Detoxification method for edible fungus strain

A technology of edible fungi and strains, applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of unstable traits, difficulty in maintaining good traits for a long time, and restrictions on the production and development of edible fungi, so as to achieve enhanced stress resistance, The effect of improving the survival rate and reducing the incidence rate

Inactive Publication Date: 2013-02-06
SICHUAN PROVINCE QINGCHUAN COUNTY CHUANZHEN INDAL
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the field of edible fungi production, in the process of popularizing and applying newly selected edible fungus strains, due to their vulnerability to viruses and germs, their properties are unstable, easy to degenerate, and aging, and it is difficult to maintain good properties for a long time. The degradation of strains Seriously restricted the development of edible fungi production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The detoxification of Pleurotus ostreatus strain Huaping No. 6, the specific operation includes the following steps:

[0024] First loop:

[0025] (1) Use 20 mm × 200 mm test tubes and 90 mm petri dishes to prepare PDA medium, add 3 drops of chloramphenicol with a concentration of 0.25-0.5% to each test tube, and add chloramphenicol with a concentration of 0.25-0.5% to each petri dish Add 6 drops of chloramphenicol to make it evenly adhere to the surface of the culture medium. Under sterile conditions, routinely inoculate Pleurotus ostreatus strains to be detoxified, and cultivate at 30°C or at room temperature.

[0026] (2) When the hyphae grow to nearly 1 / 2, insert the secondary species. The second-class species is conventionally produced, and the formula is: 2500 grams of cottonseed shells, 50 grams of superphosphate, 50 grams of gypsum powder, 15 grams of urea, and 1.5 grams of streptomycin. The mouth of the bottle should be slightly full of base material, leavin...

Embodiment 2

[0041] Applied to the detoxification of black fungus Xinke No. 5 bacteria, all steps are the same as in Example 1, the only difference is that the bacteria to be detoxified is Auricularia no. Significantly improved, the seed production survival rate of original species and cultivated species increased from 75% to 95%, fruit body quality also improved significantly, and the incidence rate decreased by 65%.

Embodiment 3

[0043] Applied to the detoxification of mushroom 9608 strains, all steps are the same as in Example 1, the only difference is that the strains to be detoxified are mushroom 9608 strains, and the results show that after detoxification, the strains' ability to resist miscellaneous bacteria is significantly improved. The seed production survival rate of species and cultivated species increased from 70% to 97%, the fruiting body was thicker, the block shape was enlarged, the quality was improved, and the incidence rate was reduced by 70%.

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PUM

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Abstract

A detoxification method for an edible fungus strain comprises the following steps: making a potato dextrose agar (PDA) culture medium in a conventional method, utilizing chloramphenicol to process the surface of the culture medium, adding the to-be-detoxified strain, and performing conventional culture; when hyphae grow to 1 / 2, adding a secondary culture medium in the conventional method to perform culture, extracting a primordium tissue to add the PDA culture medium, and performing culture in the conventional method; when the hyphae grow to 1 / 2, picking mycelia at the 2-3mm positions of tips of the hyphae, adding the PDA culture medium, and performing culture in the conventional method; adopting a primordium separation method and a tip separation method to perform cyclic operation for 2-4 times, and achieving the aim of detoxification. Original biological characteristics and production traits of the detoxified strain can be recovered completely, stress resistance and disease resistance are obviously reinforced, the morbidity is reduced by over 60% in comparison with conventional strains, the seed production survival rate of original seeds and cultivated seeds can be improved to over 95%.

Description

technical field [0001] The invention relates to a detoxification method for edible fungus strains. Background technique [0002] In the field of edible fungi production, in the process of popularizing and applying newly selected edible fungus strains, due to their vulnerability to viruses and germs, their properties are unstable, easy to degenerate, and aging, and it is difficult to maintain good properties for a long time. The degradation of strains Seriously restricted the development of edible mushroom production. In the production, there is an urgent need for a detoxification and rejuvenation technology of edible fungus strains, which can inhibit the degradation of the strains, maintain and restore the original excellent biological characteristics and production traits of the strains. Contents of the invention [0003] The problem to be solved by the present invention is to provide a detoxification method for edible fungus strains. [0004] A detoxification method fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 左万军文春贵
Owner SICHUAN PROVINCE QINGCHUAN COUNTY CHUANZHEN INDAL
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