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Preparation method of composite hydrogel dressing for repairing skin injury

A composite hydrogel and skin wound technology, applied in the application field of biomedical materials, can solve problems such as secondary injury, adhesion of wound secretions, and accelerated wound healing

Inactive Publication Date: 2013-01-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional gauze dressings can isolate the external environment to a certain extent, they cannot accelerate wound healing, and because of adhesion with wound secretions, it is easy to cause secondary damage during dressing changes

Method used

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  • Preparation method of composite hydrogel dressing for repairing skin injury
  • Preparation method of composite hydrogel dressing for repairing skin injury
  • Preparation method of composite hydrogel dressing for repairing skin injury

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Add 600mg of hyaluronic acid and 60mg of sodium hydroxide into 10ml of deionized water, stir to dissolve. At the same time, a 10 mg / ml collagen solution was prepared for use. Prepare 0.25g / ml sodium trimetaphosphate aqueous solution, heat and stir until completely dissolved. Add type I collagen solution to the hyaluronic acid aqueous solution, the amount of collagen is 5% of the hyaluronic acid mass, then add 0.5ml sodium trimetaphosphate aqueous solution, and cross-link in the mold at room temperature. Afterwards, the cross-linked product was washed with deionized water to remove the lye on the surface, and then soaked in deionized water for 6 hours to completely remove the excess cross-linking agent and alkali. After washing, store in a 4°C refrigerator.

example 2

[0015] The prepared composite hydrogel dressing was evaluated biologically, and pure hyaluronic acid hydrogel was used as the blank control.

[0016] 1. Experimental method

[0017] (1) Determination of swelling ratio:

[0018] The completely dried hydrogel film was cut into uniform-sized samples and weighed. Then, the samples were placed in a twelve-well plate at room temperature, and each sample was prepared in triplicate, and an appropriate amount of phosphate buffered saline (PBS, pH 7.4) was added to each well. At a set time interval, the sample is taken out of the solution, and the excess water on the surface of the sample is gently wiped with a damp paper towel, and then its weight is weighed.

[0019] The formula for calculating the swelling ratio of the hydrogel film: swelling ratio=(the weight of the hydrogel dry film after absorbing water-the weight of the hydrogel dry film) / the weight of the hydrogel dry film

[0020] (2) Determination of in vitro degradation pe...

example 3

[0030] The prepared composite hydrogel dressing was used for animal experiments on skin wound repair. The pure hyaluronic acid hydrogel was used as the positive control, and the blank control group did not use any dressing.

[0031] 1. Experimental method

[0032] The wound repair model was constructed with 18-22g mice, divided into male and female, and randomly divided into groups. Firstly, 20% urethane was used as anesthesia for experimental mice, and the dose was 0.01 ml / g. The mice were anesthetized by intraperitoneal injection. After the anesthetic was injected for 10 minutes and the mice were in an anesthetized state, the hair on the back of the experimental mouse was cut off. The area to be cut could be determined according to the size of the wound. After cutting, disinfect the hairless area of ​​the mouse with alcohol cotton, and disinfect surgical scissors, tweezers and other surgical instruments at the same time. The clipped skin on the back of the mouse was picked...

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Abstract

The invention belongs to the application field of biological medical material, and particularly relates to a preparation method of a composite hydrogel dressing for repairing a skin injury. The composite hydrogel dressing is a hydrogel dosage form, is flexible and smooth in texture, and has good water absorption and low degradation property. The composite hydrogel dressing is prepared through the following steps of: carrying out chemically cross-linking on hyaluronic acid and collagen type I in an alkaline solution by using sodium trimetaphosphate as a cross-linking agent, washing a cross-linking product through deionized water and soaking, and removing the residual cross-linking agent and alkali. The composite hydrogel dressing can be used for rapidly prompting the injury to cure, and can be applied to repairing various types of skin injuries, such as burn and mechanical injury.

Description

technical field [0001] The invention relates to a preparation method of a composite hydrogel dressing for skin wound repair. It belongs to the application field of biomedical materials. Background technique [0002] Human beings have been looking for ways to promote wound repair. As a wound covering, dressings can temporarily protect the wound and prevent infection, provide a favorable microenvironment for wound healing, and assist wound healing. For a long time, traditional gauze dressings have been used for the longest time and have the highest market share. Although traditional gauze dressings can isolate the external environment to a certain extent, they cannot accelerate wound healing, and because of adhesion with wound secretions, it is easy to cause secondary damage during dressing changes. Therefore, it is of great significance to develop a new type of wound dressing. [0003] This patent aims to simulate the composition and structure of the extracellular matrix i...

Claims

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Application Information

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IPC IPC(8): A61L15/32
Inventor 陈荆晓黄梅施淑贞席丽丽丁广霞郑军浩曹莹邓超陈敬华
Owner JIANGNAN UNIV
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