Avermectin nanometer microemulsion and preparation method thereof

A technology of abamectin and microemulsion, which is applied in the field of abamectin nano-microemulsion and its preparation, and can solve the problems of poor dispersion of active ingredients and low bioavailability of no nano-sized abamectin microemulsion. and other problems, to achieve the effects of high dispersibility and bioavailability, uniform particle size, and high nematocide activity

Inactive Publication Date: 2013-01-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the main dosage form of abamectin currently used in actual production is emulsifiable concentrate, and abamectin emulsifiable concentrate has some problems that are not conducive to application: first, it uses a large amount of organic solvents to cause great pressure on the environment; second 1. Its active ingredients have poor dispersion, large particles, weak permeability, and low bioavailability; thirdly, it is a biologically derived pesticide with poor stability in the environment. In addition to being prone to photodegradation, it is also affected by biological enzymes and other factors. degradation
However, the preparation of nano-abamectin microemulsions has technical problems such as complex preparation process and difficulty in selecting suitable nanomaterials to achieve high drug efficacy. At present, there are no research reports on nano-abamectin microemulsions.

Method used

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  • Avermectin nanometer microemulsion and preparation method thereof
  • Avermectin nanometer microemulsion and preparation method thereof
  • Avermectin nanometer microemulsion and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1) Put 0.95 mL of triolein into a 10 mL test tube, heat to 60°C, take 0.5 g of avermectin powder, stir in triolein to obtain solution A;

[0039] 2) Take 15 mL of propanol in a 100 mL beaker, slowly add 1 g of α-butyl cyanoacrylate while stirring, then add solution A into it to obtain mixed solution B;

[0040] 3) Take a 100 mL round bottom flask, add 15 mL of deionized water and 1 mL of octylphenol polyoxyethylene ether (OP-10), place it on an electromagnetic stirrer, add the mixed solution B dropwise while stirring, and the dropwise addition is complete Stir for 60 min to obtain a mixture C;

[0041]4) Add 62 mL of deionized water to a 100 mL round bottom flask, heat to 60°C and add 0.1 g of sodium dodecyl sulfate (SDS), 0.2 g of dextran and 5 mL of octylphenol polyoxyethylene ether in sequence (OP-10), to get the mixture D;

[0042] 5) Put it on an electromagnetic heating stirrer, after heating to 60°C, drop liquid C into liquid D under stirring, stop heating afte...

Embodiment 2

[0044] Get the abamectin nano microemulsion prepared by a small amount of the above-mentioned embodiments of the present invention and the conventional preparation of abamectin as contrast respectively, after diluting 10 times with distilled water respectively, measure the abamectin prepared in embodiment 1 with laser particle size analyzer The nano-equivalent particle size of vermectin is 107.5±18.84nm, and the equivalent particle size of the conventional preparation of abamectin is 2171.4±24.33nm ( figure 2 ). Show under transmission electron microscope, the abamectin nano microemulsion agent particle of the present invention is spherical substantially, and particle size is comparatively uniform, monodisperse, non-adhesive, and diameter is about about 100nm ( image 3 A) The particles of conventional formulations of abamectin that have not been modified by nanometer formulations stick together in a string or network, and the particle size is not uniform ( image 3 B).

Embodiment 3

[0045] The encapsulation efficiency of embodiment 3 abamectin nano microemulsion

[0046] Utilize high performance liquid chromatography to the encapsulation efficiency of the abamectin nano microemulsion prepared by the present invention, abamectin measuring condition: mobile phase is methanol: water=85: 15, detection wavelength is 286 nm, and chromatographic column is C18 Reverse-phase chromatographic column (Agilent, USA), 125×4 mm; flow rate of mobile phase was 1 mL / min, injection volume was 10 μL, and column temperature was room temperature. The measurement result shows that the encapsulation rate of the abamectin nano microemulsion prepared in embodiment 1 is 63.15 ± 3.64%, shows that most of the medicament particles of the abamectin nano microemulsion of the present invention are encapsulated by PBCA to form micro capsule.

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Abstract

The invention discloses an avermectin nanometer microemulsion and a preparation method and application thereof. The avermectin nanometer microemulsion comprises following constitutes according to percent by weight: 0.5 percent of avermectin, 1 percent of medicine carrier, 0.95 percent of cosolvent, 15 percent of solvent, 6 percent of surfactant A, 0.1 percent of surfactant B, 0.2 percent of stabilizer and 76.25 percent of water. The prepared avermectin nanometer microemulsion is basically of a spherical shape, is uniform in particle size, is singly dispersed, cannot be adhered, and has high dispersion performance and biological availability, and the technological blank of the avermectin nanometer microemulsion is filled.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to an avermectin nano-microemulsion and a preparation method thereof. Background technique [0002] Avermectin (AVM for short) is a new type of actinomycete - Streptomyces avermitilis isolated from the soil collected from the Kawana area of ​​Ito City, Shizuoka Prefecture, Japan in cooperation with Kitasato University, Japan and Merck & Co. Strtptomyces avermitilis MA-4680, obtained by separation, purification and extraction of the product after fermentation, its basic structure is a sixteen-membered macrolide diglycoside compound (Shen Yinchu et al., 1994; Putter et al., 1981; Egerton, 1979). Abamectin has the characteristics of wide insecticidal spectrum, novel structure, high efficiency, safety, low residue, and good environmental compatibility (Lasota, 1991). Abamectin prevents pests and mites mainly through stomach poisoning and contact killing, with...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N25/04A01P5/00
Inventor 谢辉齐振华徐春玲
Owner SOUTH CHINA AGRI UNIV
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