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Pseudomonas and purpose of pseudomonas

A Pseudomonas and application technology, applied in the field of Pseudomonas and its application, can solve the problems of high temperature resistance, narrow salt tolerance range, and few types of beneficial strains, etc., to achieve fast growth, improve water quality, and facilitate cultivation Effect

Active Publication Date: 2013-01-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 20th century, with the rapid increase of population and the rapid development of social economy, people's demand for aquatic products has greatly increased, and the traditional aquaculture model can no longer meet people's demand for aquatic products.
However, the microecological preparations currently used have defects such as few types of beneficial bacteria, low number of effective viable bacteria, high temperature resistance, and narrow salt tolerance range, which cannot fully meet the needs of production practice and restrict the use of microecological preparations in aquaculture water quality. Purification application

Method used

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  • Pseudomonas and purpose of pseudomonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1, the acquisition of Pseudomonas stutzeri SC221-M, the following steps were carried out in sequence:

[0017] 1) Screening of bacteria

[0018] Take 10 grams of bottom mud from the grass carp breeding pond in the suburbs of Hangzhou, add 100ml Zijingang Qizhen Lake water of Zhejiang University, 5mg feed (conventional grass carp feed) and 35mg sodium citrate, mix well, add nitrite nitrogen (concentration is 5mg / L). Samples were taken every 24 hours to qualitatively detect the degradation of nitrite nitrogen. When the nitrite nitrogen is completely degraded, take a small amount for preservation, and then add the rest of the nitrite nitrogen; that is, continue to add nitrite nitrogen to the completely degraded mixed solution (the concentration starts from 5mg / L ), until the maximum concentration of nitrite nitrogen that the bacteria can degrade is determined.

[0019] Qualitative detection of nitrite nitrogen: Spot the culture medium with a nitrite chromogenic...

Embodiment 2

[0036] Embodiment 2, the preparation method of Pseudomonas stutzeri (Pseudomonas stutzeri) SC221-M bacterial powder, carry out the following steps successively:

[0037] Pseudomonas stutzeri (Pseudomonas stutzeri) SC221-M was inoculated in LB liquid medium (peptone 10g / L, NaCl 10g / L, yeast extract 5g / L, and the rest were water; the NaOH solution with a concentration of 10mol / L was used to adjust pH7.0, sterilized at 121°C for 20 minutes), cultured at 30°C for 20 hours, centrifuged to take the sludge, dried at a constant temperature of 60°C to constant weight, and mixed with cornstarch at 4:1 (that is, cornstarch and dried The weight ratio of the bacteria slime is 4:1) to make dry bacteria powder, and the number of live bacteria per gram of dry bacteria powder is 10 8 cfu.

Embodiment 3

[0038] Embodiment 3, Pseudomonas stutzeri (Pseudomonas stutzeri) SC221-M is to the purifying effect of simulated wastewater

[0039] Six 1000L Erlenmeyer flasks were loaded with 500ml simulated wastewater, in which C / N was 4, ammonia nitrogen was 0.86mg / L, nitrite nitrogen was 117.72mg / L, and nitrate nitrogen was 16.74mg / L. Into the simulated wastewater according to 1×10 8 cfu / m 3 The final concentration of Pseudomonas SC221-M bacteria powder (obtained in Example 2) was added as the experimental group, and no bacteria powder was added as the control group, and each group was repeated three times. Ammonia nitrogen (NH 4 + -N), N-(1-naphthyl)-ethylenediamine spectrophotometric determination of nitrite nitrogen (NO 2 - -N), UV spectrophotometric determination of nitrate nitrogen (NO 3 - -N). Detected after 24h:

[0040] test group:

[0041] Ammonia nitrogen (NH 4 + -N) concentration: 1.09mg / L, nitrite nitrogen (NO 2 - -N) concentration: 0.06mg / L, nitrate nitrogen (...

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Abstract

The invention discloses pseudomonas with the preservation name being pseudomonas stutzeri SC221-M, the preservation unit is China Center for Type Culture Collection (CCTCC), the preservation address is Wuhan university, Wuhan, China, the preservation date is July 11, 2012, and the preservation number is CCTCC No: M2012281. The invention simultaneously discloses a purpose of the pseudomonas used for purifying culture wastewater.

Description

technical field [0001] The invention relates to a pseudomonas (Pseudomonas stutzeri) SC221-M and its application. Background technique [0002] Since the 20th century, with the rapid increase of population and the rapid development of social economy, people's demand for aquatic products has greatly increased, and the traditional aquaculture model can no longer meet people's demand for aquatic products. In the past two decades, my country's aquaculture industry has developed rapidly, and intensive high-density aquaculture has gradually become the mainstream of aquaculture. The purpose is to make use of various limited water resources to obtain better economic benefits and production. The contribution of artificial breeding to the production of aquatic products is increasing. In 2006, the total amount of aquatic products in the country was about 52.9 million tons, of which the production of aquaculture was 35.97 million tons, accounting for 68% of the total production of fishe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 李卫芬张小平郑佳佳邓斌傅罗琴梁权余东游
Owner ZHEJIANG UNIV
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