Natural L-alpha-glycero-phosphatidylcholine preparation method

Abstract

The invention belongs to the technical field of natural drug isolation and purification and relates to a natural L-alpha-glycero-phosphatidylcholine preparation method. The method includes: using acid liquor for soaking macroporous cation exchange resin, draining the acid liquor, washing with water, soaking with alkali liquor, washing with water, soaking with acid liquor again, washing with deionized water and using low-carbon alcohol for replacement; adding powder phospholipid into the low-carbon alcohol, alkalifying for reaction, leaching, washing with the low-carbon alcohol, adjusting pH to be alkalescent, decomposing, condensing, cooling, transferring into a separating funnel, standing, and separating out a lower-layer low-carbon alcohol phase; packing the powder phospholipid and the alcohol-phase resin, adsorbing alcoholysis mixed solution, washing the resin with low-carbon alcohol to remove impurities; and eluting the resin with pure water; and decolorizing with decolorizing agent, decomposing and condensing to obtain a finished product. The natural L-alpha-glycero-phosphatidylcholine preparation method has the advantages that process steps are simple, and industrial production requirements are met; resources are saved and cost is reduced; and the total yield of the finished product reaches 65.47-86.08%, the external standard content is more than 99%, and the optical purity is up to 99%.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of natural medicines, and in particular relates to a preparation method of natural L-alpha-glycerol phosphatidylcholine. Background technique [0002] Glycerol phosphatidylcholine (English name: L-α-glycerophosphorylCholine, abbreviated as L-α-GPC) is a naturally occurring water-soluble phospholipid metabolite in the human body, and it is also a precursor for the synthesis of acetylcholine and phosphatidylcholine (PC). , whose structure consists of choline, glycerol and phosphatidyl groups. L-α-GPC is a cholinesterase inhibitor, which can effectively prevent and treat cerebral ischemic stroke. It can not only improve the memory and cognitive ability of patients, but also treat Alzheimer's disease and cranial nerve injury. It can also resist muscle atrophy, resist hyperlipidemia and arteriosclerosis, and protect blood vessels, so it is widely used in industries such as medicine...

AI Technical Summary

Problems solved by technology

This patent application scheme adopts the solvent precipitation method to carry out purification, one of the shortcoming of existence, the phosphatidylcholine content of the lecithin used is more than 80% just can obtain pure glycerol phosphatidylcholine L-α-GPC, yet due to nature The content of phosphatidylcholine in soybean lecithin is only 20 ~ 23%, so in order to obtain the phosphatidylcholine of more than 80% content, just must carry out deep processing to lecithin, thereby can improve preparation cost undoubtedly; Two, because Precipitate with a large amount of non-polar solvents to remove unreacted lecithin, catalyst and fatty acid esters generated by the reaction, so the production cost is high and the operation is complicated; three, because the phosphatidylcholine content of lecithin is below 80% , and the mixture obtained from lecithin hydrolysis includes glycerol phosphatidylcholine L-α-GPC, glycerol phosphatidylserine L-α-GPS and glycerol phosphatidylethanolamine L-α-GPE, so the purity cannot be guaranteed