System and method for full-automatic patch clamp electrophysiological recording

Abstract

The invention discloses a system and a method for full-automatic patch clamp electrophysiological recording. The system comprises a core processing device, a sample moving platform, a video device, a digital to analog conversion amplifier device, an electromechanical control microoperation device, a positive and a negative pressure device and a recording electrode. According to a resistance value measured by the digital to analog conversion amplifier device and image information acquired by the video device, the electromechanical control microoperation device is moved and a valve of the positive and negative pressure device is controlled, and the optimum patch clamp recording state after cell membranes are broken under suction automatically when resistance in begohm is obtained through full-automatic operation can be acquired. The system and the method can be used for recording of irregular cells and anchorage-dependent cells, and overcome limitation of existing automatic patch clamp systems which can record suspending cells and cells with regular appearances only.

Description

technical field [0001] The invention belongs to the field of patch clamp technology, and in particular relates to a fully automatic patch clamp electrophysiological recording system and a recording method. Background technique [0002] The patch clamp technique is a research method used to record the electrophysiological properties of ion channels on the whole cell or individual cell membranes. The purpose is to provide basic research knowledge and to study the influence of cell electrical properties or small molecule drugs on the properties of ion channels on cell membranes during the development of new drugs. Provide a testing platform for the development of targeted drugs. The traditional patch clamp system for cell culture is operated manually. After the experimenter obtains meta-generation cells (such as cardiomyocytes and neurons), he raises the research object cells on glass slides, and manually moves the recording electrodes to place them on the cell body and place t...

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Problems solved by technology

However, this automatic patch clamp system has the following application limitations: (1) It can only be applied to the recording of suspension cells, so most of the recording objects are cancerous cells, and for most normal cells that need to adhere to the wall, now Some automatic patch clamp systems cannot record; (2) On the recording objects, the current patch clamp system can only record cells with flat and plump membranes, most of which are tool cells such as cancerous cells. A relatively strong cell membrane can withstand various human manipulations, but many cells with research value (such as metastatic neurons) have weak cell membranes that are easy to rupture, and the surface of the cell body is irregular, and the existing automatic patch clamp system is difficult to dispatch. Useful

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