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Potassium ion concentration detection method

A technology of potassium ion concentration and concentration, applied in the field of biomedicine, can solve problems such as large error and complicated operation

Active Publication Date: 2013-01-09
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] (5) Atomic spectrophotometry can also be used to detect potassium and sodium ions in serum, but the operation is complicated and the error is large, and it is not as simple as flame photometry

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0053] In this embodiment, the potassium ion concentration of three urine samples is verified. The actual potassium ion concentration of each urine sample is as follows: urine sample 1 is 5.84mmol / L, urine sample 2 is 22.25mmol / L, and urine sample 3 is 38.17 mmol / L. The DNA capable of forming a G-quadruplex used in this example is TTAGGGTTAGGGTTAGGGTTAGGG, and the cyanine dye used is a compound of the following formula

[0054]

[0055] 1) Prepare standard solution samples

[0056] Dissolve a certain amount of DNA sample in Tris-HCl (Tris-Na) buffer (pH8.0) containing 20mmol / L NaCl to prepare a DNA stock solution with a concentration of 100μmol / L for later use.

[0057] Take 300 μL of methanol solution with a concentration of 200 μmol / L cyanine dye, add 9.1 ml of Tris-Na buffer, and then add 600 μL of DNA solution and mix well. Divide the above sample into 10 parts on average, each sample solution is 1mL.

[0058] Take 7 samples among them, add a certain amount of Tris-N...

Embodiment 2

[0064] In this example, the potassium ion concentration of three urine samples is verified. The actual potassium ion concentration of each urine sample is as follows: urine sample 1 is 9.18mmol / L, urine sample 2 is 32.43mmol / L, and urine sample 3 is 49.57 mmol / L. The DNA capable of forming a G-quadruplex used in this example is AGGGTTAGGGTTAGGGTTAGGG, and the cyanine dye used is a compound of the following formula

[0065]

[0066] 1) Prepare standard solution samples

[0067] A certain amount of DNA sample was dissolved in Tris-HCl (Tris-Na) buffer (pH6.2) containing 40mmol / L NaCl to prepare a DNA stock solution with a concentration of 500μmol / L for later use.

[0068] Take 300 μL of methanol solution with a concentration of 200 μmol / L cyanine dye, add 9.14 ml Tris-Na buffer, and then add 360 μL of DNA solution and mix well. Divide the above sample into 10 parts on average, each sample solution is 0.98mL.

[0069] Take 7 samples among them, add a certain amount of Tris-...

Embodiment 3

[0075] In this embodiment, the potassium ion concentration of three urine samples is verified. The actual potassium ion concentration of each urine sample is as follows: urine sample 1 is 9.82mmol / L, urine sample 2 is 20.35mmol / L, and urine sample 3 is 78.26 mmol / L. The DNA capable of forming a G-quadruplex used in this example is TTAGGGTTAGGGTTAGGGTTAGGGTTAGGG, and the cyanine dye used is a compound of the following formula

[0076]

[0077] 1) Prepare standard solution samples

[0078] A certain amount of DNA sample was dissolved in Tris-HCl (Tris-Na) buffer (pH 7.5) containing 100 mmol / L NaCl to prepare a DNA stock solution with a concentration of 500 μmol / L for later use.

[0079] Take 0.4 mL of methanol solution with a concentration of 200 μmol / L cyanine dye, add 4.2 mL of Tris-Na buffer, then add 0.4 mL of DNA solution and mix well. Divide the above sample into 10 parts on average, each sample solution is 0.5mL.

[0080] Take 7 samples among them, add a certain amo...

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Abstract

The invention relates to a potassium ion concentration detection method. The characteristic for forming the conversion of a G-four chain deoxyribonucleic acid (DNA) structure by controlling potassium ions and the characteristic for recognizing the conversion of the G-four structure through cyanine dye supramolecular aggregate are utilized, a sample to be tested is placed into DNA and cyanine dye mixed solution capable of forming the G-four chain, and the concentration range of the potassium ions in the sample can be semiquantitatively judged according to the color of the solution. Due to adopting the method, the visible detection of the potassium ion concentration can be realized, and detection test paper can be developed. A reagent is simple in ingredients, the reaction process is simple, no special or additional instrument is needed, the detection cost is low, and the method is convenient for being popularized to use in the industry.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a potassium ion concentration detection method. Background technique [0002] Potassium in the human body is the main cation for maintaining the physiological activities of cells. It is necessary to maintain the normal osmotic pressure and acid-base balance of the body, participate in the metabolism of sugar and protein, and ensure the normal function of neuromuscular. Its content is an important indicator of human physiological activities. The levels of potassium ions in urine and serum can be clinically used to diagnose some kidney and heart diseases. [0003] Under normal circumstances, the potassium ion concentration of the human body has a reasonable reference range, such as in serum: 3.5-5.5mmol / L; in urine: 25-125mmol / 24h. When the potassium ion is higher than the reference value, hyperkalemia is manifested. The main reasons are: acute renal failure, severe hemolysi...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/52
CPCC09B23/06C09B23/0008
Inventor 唐亚林孙红霞杨千帆尚倩姜薇盖伟向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI
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