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Optimization process for factory production of hypsizygus marmoreus by adopting liquid strains

A liquid strain, factory-like technology, applied in application, horticulture, fertilizer mixture, etc., can solve problems such as difficulty in forming primordia, and achieve the effect of shortening the growth cycle

Inactive Publication Date: 2012-12-26
SHANDONG ZHENGHAN BIO TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after inoculation with liquid strains, there are no old strains in the bacterial bottle, and the original base needs to be formed directly on the surface of the cultivation material, which is difficult to form.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. The weight percentage of the original seed formula is: 32% sawdust, 30% corn cob, 13% rice bran, 24% bran, and 1% lime. After mixing with water, the pH is 8.5 and the water content is 61.4%. The bottling weight of the cultivation bottle of 1100ml is 760 / bottle, and 4 holes are punched, and the diameter is 12mm. After punching, the material surface is 10mm away from the bottle mouth.

[0018] 2. After the bacteria bottle is sterilized and cooled to room temperature, inoculate the liquid bacteria. The amount of inoculation of liquid bacteria is 20ml per bottle of 1100ml cultivation bottle. After the inoculation, the cells were sent to the culture room for cultivation. The environmental control parameters of the culture room are: the temperature is controlled at 21-23 °C, the CO in the air 2 The concentration is controlled at 3000ppm, and the air humidity is controlled at 60% to 80%.

[0019] 3. After 70 days of culture, select the original species that are free fro...

Embodiment 2

[0024] 1. The weight percentage of the original seed formula is: 20% wood chips, 38% corncobs, 20% rice bran, 20% bran, and 2% lime. After mixing with water, the pH is 8.2 and the water content is 62.3%. The bottling weight of the cultivation bottle of 1100ml is 760 / bottle, and 3 holes are punched with a diameter of 12mm. After punching, the material surface is 12mm away from the bottle mouth.

[0025] 2. After the bacteria bottle is sterilized and cooled to room temperature, inoculate the liquid bacteria. The amount of inoculation of liquid bacteria is 15ml per bottle of 1100ml cultivation bottle. After the inoculation, the cells were sent to the culture room for cultivation. The environmental control parameters of the culture room are: the temperature is controlled at 21-23 °C, the CO in the air 2 The concentration is controlled at 5000ppm, and the air humidity is controlled at 60% to 80%.

[0026] 3. After 73 days of culture, select the original species that are free fr...

Embodiment 3

[0031] 1. The weight percentage of the original seed formula is: 20% wood chips, 33% corncobs, 20% rice bran, 25% bran, and 2% lime. After mixing with water, the pH is 8.0 and the water content is 61.0%. The bottling weight of the cultivation bottle of 1100ml is 760 / bottle, and 3 holes are punched, the diameter is 14mm, and the material surface is 10mm away from the bottle mouth after punching.

[0032] 2. After the bacteria bottle is sterilized and cooled to room temperature, inoculate the liquid bacteria. The amount of inoculation of liquid bacteria is 20ml per bottle of 1100ml cultivation bottle. After the inoculation, the cells were sent to the culture room for cultivation. The environmental control parameters of the culture room are: the temperature is controlled at 21-23 °C, the CO in the air 2 The concentration is controlled at 4000ppm, and the air humidity is controlled at 60% to 80%.

[0033] 3. After 80 days of cultivation, select the original species that are fr...

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PUM

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Abstract

The invention relates to the field of edible fungus culture, in particular to an optimization process for factory production of hypsizygus marmoreus by adopting liquid strains. According to the optimization process, the proper stock seed and culture seed recipes are selected, the water content, the pH, the bottle weight and the inoculum size of the culture materials are controlled within a proper range, in addition, the good-ventilation and wide-distribution-range perforation mode is matched, and good environment is provided for the fixed planting growth of the liquid strains on the stock seed culture materials and the germination and the growth of the stock seeds in the culture materials, the stock seed growth period is greatly shortened, the stock seed full bottle time is only 25 to 30 days, and the solid seed inoculation at least needs 45 days.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to an optimized process for industrial production of shimeji mushrooms using liquid strains. Background technique [0002] Liquid strains are edible fungus strains in liquid form produced by submerged culture (liquid fermentation) technology in biological fermenters using liquid culture medium. Liquid strains are relative to the physical properties of solid strains (sawdust, cotton hulls, wheat bran, branches, etc.). Liquid strains have incomparable advantages over solid strains: fast seed production, strong vitality, high purity, anti-pollution, shortened cultivation cycle, and low cost. [0003] At present, my country's industrialized production of shimeji mushrooms generally adopts a three-level strain breeding system, that is, the mother species is used to produce the original species, and the original species is used to produce the cultivated species, and the cultivate...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 盛岩赵磊李波张红霞王艳霞蔡王涛董孝刚
Owner SHANDONG ZHENGHAN BIO TECH CO LTD
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