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Growth-promoting rhizobacteria SXH-2 and application thereof

A SXH-2, rhizosphere growth-promoting bacteria technology, applied in the application, bacteria, biocide and other directions, can solve the problems of reduced utilization rate, damage to the soil environment, limited yield of chives, etc., to promote phosphorus absorption and improve stress resistance. sex, reducing the effect of massive accumulation

Inactive Publication Date: 2013-11-20
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, planting leeks requires a large amount of chemical fertilizers, which not only affects the quality of leeks, but also damages the soil environment, and it is a well-known fact that with the extensive use of chemical fertilizers, their utilization rate continues to decrease
This shows that it is limited to increase the yield of leeks only by increasing the application of chemical fertilizers in large quantities.

Method used

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  • Growth-promoting rhizobacteria SXH-2 and application thereof
  • Growth-promoting rhizobacteria SXH-2 and application thereof
  • Growth-promoting rhizobacteria SXH-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Isolation and identification of Klebsiella oxytoca SXH-2

[0039] 1. Isolation of Klebsiella oxytoca SXH-2

[0040] The separation of Klebsiella oxytoca SXH-2 includes sampling, screening and purification. The specific methods are as follows:

[0041] 1.1 Sampling

[0042] Screened from the rhizosphere soil of leeks. The specific test leeks and soil were taken from Shuangjing Town, Hulan District, Harbin City, Heilongjiang Province (The physical and chemical properties of the soil are as follows: pH 8.15, total salt content 1.83%, and alkaline hydrolysis nitrogen 36mg / kg , Available phosphorus 8mg / kg, quick-acting 132mg / kg, organic matter 2.69%). Put leeks and plant rhizosphere soil into a clean fresh-keeping bag prepared in advance, and bring them back to the laboratory for planting to be tested.

[0043] Weigh 1g of rhizosphere soil sample in an Erlenmeyer flask containing 50mL PAF culture solution, and culture it with shaking (200r / min) at room temperature (21±1℃)...

Embodiment 2

[0061] Example 2. Determination of ACC Deaminase Activity of CGMCC No. 6297

[0062] The test strains were cultured overnight in TSB culture medium (tryptone 17.0g, soybean peptone 3.0g, NaCl 5.0g, glucose 2.5g, K2HPO42.5g, distilled water 1000mL, pH 7.5) overnight, and the bacteria were collected by centrifugation at 4°C. Bacterial cells were washed three times with DF culture medium, resuspended in ADF culture medium, cultured with shaking at room temperature (21±1℃) for 2 days, then collected by centrifugation at 4℃ and used 0.1mol·L -1 Tris-HCl buffer (pH=7.6) washed and centrifuged three times, resuspended in 600μL 0.1mol·L -1 To Tris-HCl buffer (pH=8.0), add 30μL of toluene and quickly shake for 30s to disrupt the cells, transfer 200μL of cell extract containing toluene to a 1.5mL centrifuge tube, add 20μL 0.5mol·L -1 Mix the ACC well, and do a blank test without adding ACC at the same time, incubate at 30°C for 15 minutes. Add 1mL 0.56mol·L -1 HCl, 16000g centrifugation for...

Embodiment 3

[0067] Example 3 Determination of CGMCC NO.6297 Growth Hormone IAA Synthesis Content

[0068] The test strain Klebsiella oxytoca SXH-2 was first cultured in DF medium for 2 days, and then transferred to a small amount of DF medium (containing 0, 50, 100, 200 and 500μg L-Trp·mL -1 ), continue to incubate for 2 days, sample the OD of the bacterial solution 600 Centrifuge the remaining culture medium at 8000g at room temperature, take 500μL of supernatant, add 2mL Salkowski reagent (containing 150mL H 2 SO 4 , 250mL ddH 2 O and 7.5mL 0.5mol / L FeC1 3 ), after 20min incubation in the dark at room temperature, measure the absorbance at 535nm (OD 535 ). The sterile medium is treated with the same treatment as above as a control and zeroed. Take the concentration as 0, 0.01, 0.05, 0.25, 0.5 mg·mL -1 The IAA standard solution is the same method as the standard curve. The unit of IAA content is μg·(mL·OD 600 ) -1 , IAA standard curve is paralleled twice, and the sample is repeated three t...

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PUM

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Abstract

The invention discloses a growth-promoting rhizobacteria SXH-2 with a categorical name of klebsiella oxytoca SXH-2. The strain is collected in China General Microbiological Culture Collection Center with a collection number of CGMCC No. 6297. The strain can synthesize indole acetic acid and siderophore, and has a phosphate-solubilizing effect. Therefore, the strain assists in providing indole acetic acid and iron element for plants, and assists in promoting phosphorus absorption. The strain assists in promoting plant growth. The strain has substantial promoting effect upon plant height, fresh weight and dry weight of leeks.

Description

Technical field [0001] The invention belongs to the technical field of plant growth promoting bacteria, and relates to a rhizosphere growth promoting bacteria SXH-2 and applications thereof. Background technique [0002] Leek (Allium tuberosum Rottl.ex Spr.) is a perennial root plant of the genus Allium tuberosum in the lily family. It is native to China and is cultivated throughout the country. Leeks are rich in vitamins and various minerals, and because they contain a variety of sulfides, they have a special aroma, which can increase appetite and have certain medicinal values. Its seeds and leaves can be used as medicine. It has the functions of invigorating the stomach, refreshing, antiperspirant and astringent, invigorating the kidney and yang, and consolidating essence. In Chinese medicine, leeks have a very resounding name called "aphrodisiac", and some people call leeks "intestinal herb". Not only that, but also leeks have many names. Leeks are also called stalactites, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88A01N63/02A01P21/00C12P17/10C12P1/04C05F11/08C12R1/22
Inventor 郭长虹史煦涵彭玲玲蔡洪生程园园
Owner HARBIN NORMAL UNIVERSITY
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