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A kind of bacterial strain ld‑11 capable of promoting plant growth under drought stress and its application

A technology of drought stress and culture, applied in the field of plant growth-promoting bacteria, can solve problems such as reduced utilization rate, and achieve the effects of promoting biomass in the underground part, reducing a large amount of accumulation, and improving stress resistance.

Active Publication Date: 2017-08-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year

Method used

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  • A kind of bacterial strain ld‑11 capable of promoting plant growth under drought stress and its application
  • A kind of bacterial strain ld‑11 capable of promoting plant growth under drought stress and its application
  • A kind of bacterial strain ld‑11 capable of promoting plant growth under drought stress and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Isolation and Identification of Burkholderia LD-11(Burkholderia cepacia)

[0043] 1. Isolation of Burkholderia LD-11 (Burkholderia cepacia)

[0044] The isolation of Burkholderia LD-11 (Burkholderia cepacia) includes three steps of sampling, enrichment, primary screening, and secondary screening, as follows:

[0045] 1.1 Sampling

[0046] Sampling was taken from the soil and wastewater seriously polluted by heavy metals such as mining areas, sewage treatment plants, electroplating plants, and auto repair plants in various cities, counties, and townships in Guangxi Province, put them into clean sampling bags (bottles), marked them, and stored them at 4 °C Store in the refrigerator for later use.

[0047] 1.2 Enrichment and primary screening

[0048] Weigh 10g of collected samples into 100ml containing [Cu 2+ ]=0.5mmol / L (CuSO 4 ·5H 2 O) in Luria-Bertani (LB) liquid medium, 37° C., 200 rpm, shake culture for 24 hours. Then take 1ml of the bacterial suspension and spr...

Embodiment 2

[0065] Determination of ACC deaminase yield of Burkholderia cepacia LD-11

[0066] The test strain was cultured in 10ml LB liquid medium at 30°C and 200rpm for 24h with shaking, and centrifuged at 6000rpm for 10min at room temperature to collect the bacteria. The precipitate was washed with 0.1M Tris-HCl buffer solution (pH=7.6), and the bacterial cells were collected by centrifugation at 10000 rpm. Resuspend in 600μL 0.1mol / L Tris-HCl buffer (pH=8.5), add 30μL toluene and shake rapidly for 30s to break the cells, transfer 200μL cell extract containing toluene to a 1.5ml centrifuge tube, add 20μL 0.5mol / L ACC, mix well, and do a blank test without ACC at the same time, and incubate at 30°C for 30min. Add 1ml of 0.56mol / L HCl, centrifuge at 10,000rpm for 5min, take 1ml of the supernatant into a test tube, add 20μL of 0.56mol / L HCl, shake fully, then add 300μL of 2mol / L HCl (with a final concentration of 0.2% (W / v) 2,4-dinitrophenylhydrazine), incubate at 30°C for 30min, the...

Embodiment 3

[0069] Determination of Growth Hormone IAA Synthesis Content of Burkholderia cepacia LD-11

[0070] The test strain Burkholderia LD-11 (Burkholderia cepacia) was cultured in LB medium (containing 0.5mg / mL tryptophan) at 37°C and 150rpm for 24 hours with shaking, and samples were taken to test the OD of the bacteria solution. 600 , when OD 600 When =0.5, the bacterial cells were collected. Take 2ml supernatant, add 2ml Salkowski reagent (containing 150ml concentrated sulfuric acid, 250ml ddH 2 O and 7.5ml 0.5mol / L FeCl 3 ), after 20 min of dark color development at room temperature, the absorbance was measured at a wavelength of 535 nm. The sterile medium was treated the same as above and set to zero as a control. Use IAA standard substances with concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80 μg / mL to make a standard curve in the same way (see image 3 ). The unit of IAA content is μg / mg FW.

[0071] According to the calculation and detection results of the standard...

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Abstract

The invention belongs to the technical field of plant growth-promoting bacteria, and relates to a bacterial strain LD‑11 capable of promoting plant growth under drought stress and an application thereof. A bacterial strain LD-11 that can promote plant growth under drought stress disclosed by the present invention is named Burkholderia LD-11 (Burkholderia cepacia) taxonomically, and was preserved in Chinese Type Culture on June 6, 2014. The depository center, the deposit number is CCTCC M2014246. The bacterium can synthesize ACC deaminase, reduce the damage of high levels of ethylene caused by drought stress to plants, and can also synthesize indole acetic acid and siderophores, providing exogenous indole acetic acid and iron elements for plants, thus promoting plant growth. The role of growth.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and relates to a bacterial strain LD-11 capable of promoting plant growth under drought stress and an application thereof. Background technique [0002] Soil drought is one of the most important environmental factors restricting crop growth, development and yield improvement. According to conservative estimates, only in the tropics, the annual loss of corn caused by drought is at least 20 million tons, and the loss rate is at least 17%. In recent years, with climate change, the trend of global drought has become more and more serious. Therefore, the aggravation of drought has seriously affected the potential of crop yield and restricted the development of agricultural economy. How to maximize crop yield potential under dry farming conditions is an urgent problem facing scientists. Using the interaction between crops and microorganisms, screening plant growth-promoting b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 樊宪伟李有志胡海洋黄桂海
Owner GUANGXI UNIV
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