2-phenylethanol-producing yeast strain, its culturing method and application
A culture method and strain technology, applied in the field of microorganisms, can solve the problem of high synthesis cost of 2-phenylethanol, achieve high tolerance and increase yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] A strain of Saccharomyces cerevisiae (Saccharomyces cerevisiae) WHH6 strain was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on December 20, 2010, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, The deposit number is CGMCC No.4491.
[0044] Saccharomyces cerevisiae (Saccharomyces cerevisiae) WHH6 strain has typical characteristics of Saccharomyces cerevisiae, the colony is white, with rounded edges and a slightly raised center, such as figure 1 As shown; the cells are nearly round, with an average size of 15-21 μm×14-16 μm (length×width), such as figure 2 Shown; Fermentation produces a strong aroma, and the ethanol production can reach 9.8% (v / v). The single-phase single strain can ferment and produce 2-phenylethanol up to 5.4003g / L in the optimized medium (Table 1). It can be used as 2-phenylethanol, wine and feed yeast productio...
Embodiment 2
[0046] The culture method of Saccharomyces cerevisiae (Saccharomyces cerevisiae) WHH6 bacterial strain, the steps are as follows:
[0047] (1) Pick 1-2 rings of Saccharomyces cerevisiae WHH6 from the inclined surface of the test tube and insert it into the liquid seed medium, and activate and shake it for 18-22 hours under the conditions of 28-30°C and 120-160r / min to obtain activated liquid seeds ;
[0048] (2) Inoculate the liquid seeds prepared in step (1) into the liquid fermentation medium at an inoculum size of 8-12% (v / v), and ferment and cultivate at 28-30°C and 120-160r / min. After culturing for 24 h, samples were taken every 12 h to determine the OD of the fermentation broth 660 value and 2-phenylethanol content in the supernatant (results such as Figure 5 shown), after 40 h of fermentation culture, the content of 2-phenylethanol in the fermentation supernatant and the OD of the bacterial liquid were measured every 4 h 660 value (results such as Figure 6 shown),...
Embodiment 3
[0085] The application of Saccharomyces cerevisiae (Saccharomyces cerevisiae) WHH6 strain in the preparation of 2-phenylethanol, the steps are as follows:
[0086] (1) Pick 2-ring Saccharomyces cerevisiae WHH6 from the slant of the test tube and insert it into the liquid seed medium, and activate and shake it for 20 hours at 28°C and 120r / min to obtain activated liquid seeds;
[0087] (2) Inoculate the liquid seeds prepared in step (1) into the liquid fermentation medium at an inoculum size of 8% (v / v), and culture and ferment them at 28°C and 120r / m for 24 to 40 hours respectively. Add 10% sterilized oleic acid in the volume of the liquid fermentation medium at the time point, continue to cultivate at 28°C and 120r / min until the total time is 56h, centrifuge at 6000r / min for 10min, take the oil phase, and obtain 2- phenethyl alcohol.
[0088] Preferably according to the present invention, the components of the liquid seed medium in the step (1) are as follows:
[0089] Yeas...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com