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Extraction method for microalgae grease

A technology of microalgae oil and extraction method, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of ineffective oil extraction, poor oil extraction effect, and difficult industrial development, etc., to speed up the preparation and development and utilization speed, The extraction speed is fast and the effect of industrialized scale-up production is realized

Inactive Publication Date: 2012-12-12
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although supercritical CO 2 The method can greatly shorten the extraction time and reduce the amount of extractant used, but supercritical CO 2 The method is plagued by factors such as complex operation, expensive equipment, and high cost, making industrial development difficult
The organic solvent method directly utilizes the characteristics that microalgae oil is easily soluble in organic solvents, and can be extracted by selecting a suitable organic solvent, but the oil extraction effect of this method is poor, because the cell crushing ability is poor, and the oil in the cells cannot be effectively extracted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Sample preparation: Centrifuge (10000r / m, 5min) to collect Scenedesmus sp. cells, and then use a vacuum freeze dryer to freeze-dry to obtain dry algae powder;

[0023] (2) Ultrasonic pretreatment: Weigh 5g of dry algae powder into a 250mL conical flask, add 100mL of 95% alcohol, seal it, place it in an ultrasonic cleaner, and process it for 30 minutes at an ultrasonic power of 360W;

[0024] (3) Soxhlet extraction: put the ultrasonically treated material into a Soxhlet extractor, extract at 99°C or in a boiling water bath for 3.5 hours (the number of siphons is 25-30 times), and then use vacuum filtration to filter and separate. The filtrate was concentrated under reduced pressure with a rotary evaporator to obtain 1.238 g of microalgae oil, and the extraction efficiency was 24.8%.

Embodiment 2

[0026] (1) Sample preparation: Centrifuge (10000r / m, 5min) to collect Chlorella sp. cells, and then use a vacuum freeze dryer to freeze-dry to obtain dry algae powder;

[0027] (2) Ultrasonic pretreatment: Weigh 5g of dry algae powder into a 250mL conical flask, add 150mL of 95% alcohol, seal it, place it in an ultrasonic cleaner, and process it for 60 minutes at an ultrasonic power of 360W;

[0028] (3) Soxhlet extraction: put the ultrasonically treated material into a Soxhlet extractor, extract at 99°C or in a boiling water bath for 3 hours (the number of siphons is 25-30 times), and then use vacuum filtration to filter and separate, and use The filtrate was concentrated under reduced pressure by a rotary evaporator to obtain 2.014 g of microalgae oil, with an extraction efficiency of 40.3%.

Embodiment 3

[0030] (1) Sample preparation: Centrifuge (10000r / m, 5min) to collect Platymonas subcordiformis cells, and then use a vacuum freeze dryer to freeze-dry to obtain dry algae powder;

[0031] (2) Ultrasonic pretreatment: Weigh 5g of dry algae powder into a 250mL conical flask, add 150mL of 95% alcohol, seal it, place it in an ultrasonic cleaner, and process it for 60 minutes at an ultrasonic power of 360W;

[0032] (3) Soxhlet extraction: put the ultrasonically treated material into a Soxhlet extractor, extract at 99°C or in a boiling water bath for 3 hours (the number of siphons is 25-30 times), and then use vacuum filtration to filter and separate, and use The filtrate was concentrated under reduced pressure by a rotary evaporator to obtain 1.825 g of microalgae oil with an extraction efficiency of 36.2%.

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Abstract

The invention discloses an extraction method for microalgae grease. The method comprises the following steps: (1) preparation of a sample: a step of collecting microalgae cells through centrifugation and then carrying out freeze drying so as to obtain dry microalgae powder; (2) ultrasonic pretreatment: a step of mixing the dry microalgae powder with alcohol and carrying out ultrasonic treatment; and (3) Soxhlet extraction: a step of adding a material obtained after treatment in step (2) into a Soxhlet extractor, carrying out extraction in a boiling water bath for 3.5 h and then carrying out filtration, separation and reduced pressure concentration so as to obtain the microalgae grease. The method provided by the invention can obviously improve preparation processes for the microalgae grease, is beneficial for realization of industrial production and can speed up preparation, development and utilization of the microalgae grease.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a simple extraction method of microalgae oil. Background technique [0002] Due to the depletion of petrochemical energy and the environmental pollution it brings, the development of renewable new energy has become a hot spot in the world today. Microalgae is not only a potential resource for the production of high value-added products and biologically active substances, but also an important raw material for biomass energy production. Compared with other energy plants, the oil content in microalgae biomass is high, and the output per unit area can reach up to More than 30 times that of terrestrial oil crops, so it is considered to be an important raw material for future bioenergy supply. [0003] At present, the preparation methods of microalgae oil mainly include supercritical CO 2 method and organic solvent method. Although supercritical CO 2 The method can greatly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 邓祥元高坤景吉靡慧
Owner JIANGSU UNIV OF SCI & TECH
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